外源硒和耐硒细菌对镉胁迫下水稻生长、生理和硒镉积累的影响

王波; 张然然; 杨如意; 石晓菁; 苏楠楠; 朱濛; 昝树婷

Effect of exogenous selenium（Se）and Se-tolerant bacterium on the growth, physiology, and Se and cadmium （Cd）accumulation in rice （Oryza sativa L.）grown under Cd stress

Received:April 17, 2020

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KeyWord:Oryza sativa L.;cadmium contamination;antagonism;selenium-tolerant bacteria

Author Name	Affiliation	E-mail
WANG Bo	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, Wuhu 241002, China Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Normal University, Wuhu 241002, China
ZHANG Ran-ran	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
YANG Ru-yi	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, Wuhu 241002, China Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Normal University, Wuhu 241002, China	yangruyi@ahnu.edu.cn
SHI Xao-jing	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
SU Nan-nan	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
ZHU Meng	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, Wuhu 241002, China Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Normal University, Wuhu 241002, China
ZAN Shu-ting	School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China Anhui Provincial Engineering Laboratory of Water and Soil Pollution Control and Remediation, Wuhu 241002, China Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, Anhui Normal University, Wuhu 241002, China

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Abstract:

Cadmium(Cd)is among the most toxic and widespread pollutants in agricultural soils in China. The antagonism between selenium(Se)and Cd has been extensively investigated in the past decades. However, the effects of Se application amount and Se-tolerant bacteria on Se and Cd accumulation in plants remain poorly understood. A pot experiment was conducted to investigate the effects of exogenous Se and inoculation with Se-tolerant bacterium Lysinibacillus macrolides strain DS15 on the growth, physiology, and Se and Cd accumulation in rice(Oryza sativa L.)grown under Cd stress. The results showed that exogenous Se increased the shoot length of rice, increased Se accumulation, and decreased the activities of superoxide dismutase(SOD)and catalase(CAT)enzymes. Low(0.5 mg·kg^-1 Na₂SeO₃)and high(2.0 mg·kg^-1 Na₂SeO₃)Se additions significantly decreased the availabilities of Cd in rhizosphere soil by 32.24% and 9.43%, respectively, thereby reducing Cd accumulation in shoot and transportation in rice. The effects of low Se treatment on the immobilization of Cd in soil and the alleviation of Cd stress were consistently superior to those of high Se treatment, while excessive Se increased Cd content in rice shoot and root under sterilization condition compared to the control. Inoculation with Se-tolerant L. macrolides increased rice biomass by 1.79 times but decreased Se contents in rice, and thus, it induced the increase of SOD and CAT activities. L. macrolides reduced the availability of Cd in rhizosphere soil by 18.46%, and thus, it decreased Cd accumulation and transportation in rice. Interaction between exogenous Se and inoculation with L. macrolides affected the accumulation and transportation of Se and Cd in rice significantly. Low Se and inoculation with L. macrolides decreased Cd uptake in rice, while high Se and inoculation with L. macrolides decreased the transportation of Cd. This study indicates that low Se and Se-tolerant bacterium can decrease the availability of Cd in soil, and thus, reduce the accumulation and transportation of Cd in rice.