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| Screening of thermophilic cellulolytic bacteria and investigation of cellulase thermostability |
| Received:August 14, 2020 |
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| KeyWord:aerobic composting;thermophilic cellulolytic bacteria;cellulase;straw |
| Author Name | Affiliation | E-mail | | JIANG Gao-fei | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | BAO Yan-zhuo | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | YANG Tian-jie | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | ZHENG Hai-ping | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | MEI Xin-lan | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | WEI Zhong | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | | | XU Yang-chun | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | ycxu@njau.edu.cn | | SHEN Qi-rong | College of Resources and Environmental Sciences/Key Laboratory of Plant Immunity/Jiangsu Provincial Key Lab of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center of Solid Organic Wastes/National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China | |
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| Abstract: |
| The aim of this study was to screen efficient cellulolytic bacteria at high temperatures(55~75℃)and evaluate the straw degradation and thermostability of cellulase. Thermophilic cellulolytic bacteria were isolated from composts enriched by a serial subcultivation in rice straw at 55, 65℃, and 75℃. Targeted strains were screened based on hydrolytic activity and degradation efficiency. The resulting bacteria were subjected to 16S rRNA sequencing and phylogenic analyses, and enzyme thermostability investigation. Bacterial strain B-7 and B-11 were screened from 13 thermophilic cellulolytic bacterial isolates owing to their high cellulolytic efficiency and the carboxymethyl cellulose activity. The 16S rRNA analysis revealed that B-7 and B-11 belong to Geobacillus stearothermophilus and Bacillus pumilus, respectively. Cellulase thermostability results showed that the cellulase activity of B-7 and B-11 first increased and then decreased at 50~90℃ with optimal enzyme activity at 55~65℃ and 70~80℃, respectively. B-7 and B-11 are efficient thermophilic cellulolytic bacteria with different temperature preferences. These two thermophilic bacteria show great potential for a potential for broad application in high temperature aerobic composting. |
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