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| Characteristics of a chlorpyrifos-degrading bacterial strain and optimization of its degradation conditions by response surface methodology |
| Received:February 27, 2020 |
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| KeyWord:chlorpyrifos;bacterial strain;degradation characteristics;single-factor experiment;response surface methodology |
| Author Name | Affiliation | E-mail | | DU Xiao-min | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | | | WANG Jin-hua | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | wjh@sdau.edu.cn | | ZHU Lu-sheng | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | | | WANG Jun | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | | | YANG Li-li | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | | | LIN Lin | College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Tai'an 271018, China | |
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| Abstract: |
| In order to obtain a bacterial strain for bioremediating chlorpyrifos contamination, a bacterial strain capable of degrading chlorpyrifos was isolated from field soil. Based on its morphological characteristics and 16S rDNA sequence analysis, the strain was identified as Bacillus and named H27. Through single-factor degradation condition experiments, the results showed that its optimal degradation time was 48 h, optimal temperature was 25℃, and optimal pH was 7.0. Localization of the chlorpyrifos-degrading enzyme was conducted, and the results showed that chlorpyrifos-degrading enzymes were mainly intracellular enzymes. In order to improve the chlorpyrifos degradation rate, three factors were further optimized using the Box-Behnken design and response surface analysis. When the initial concentration of chlorpyrifos was 25 mg·L-1, the optimal conditions were as follows:Time of 54 h, pH of 7.2, and temperature of 24℃. Under these optimal conditions, the chlorpyrifos degradation rate reached 88.96%. This study provides information for the effective treatment of chlorpyrifos-contaminated soil. |
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