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| Toxic mechanism of uranium on photosynthetic characteristics and respiratory metabolism of Vicia faba L. |
| Received:April 07, 2020 |
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| KeyWord:uranium (U);photosynthesis;respiratory metabolism;transcriptome;Vicia faba L. |
| Author Name | Affiliation | E-mail | | LIU Ze-wei | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | | | LAI Jin-long | College of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | | | LI Jun-ke | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | | | DING Feng | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | | | ZHANG Yu | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | zhangyu@swust.edu.cn | | LUO Xue-gang | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China | |
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| Abstract: |
| This study aims to explore the toxicity of uranium(U)stress on photosynthesis and respiratory metabolism in plants. In this experiment, seedlings of Vicia faba L. were treated with different concentrations of U(0, 5, 10, 15, 20 μmol·L-1 and 25 μmol·L-1)for 72 h. Physiological and biochemical analyses were conducted, and the roots of the high-concentration U treatment group and the control group were selected using RNA-seq. The results showed that under different concentrations of U stress, U rarely transferred to the aboveground part of the plant, its transfer coefficient was less than 0.01, and the excess U accumulation clearly damaged the root structure. In addition, the leaf photosynthetic efficiency of Vicia faba L. decreased, but the root activity increased under U stress. These processes are regulated by related genes. The results of transcriptome sequencing showed that there were 117 differentially expressed genes(DEGs)involved in photosynthesis and respiratory metabolism. The expression of photosynthesis-related genes was mainly down-regulated(90 DEGs, 3 up and 87 down), while that of respiratory metabolism-related genes was mainly up-regulated(27 DEGs; 23 up and 4 down). Therefore, this research shows that the photosynthesis of Vicia faba L. is inhibited and the respiration is enhanced under high-concentration U treatment. |
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