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| Construction and application of an enzyme-linked immunosorbent assay for detection of tetrabromobisphenol A |
| Received:January 17, 2020 |
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| KeyWord:TBBPA;TBBPA-DHEE;TBBPA-MHEE;monoclonal antibody |
| Author Name | Affiliation | E-mail | | HUANG Zhe | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | SHAO Jie | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | ZENG Kun | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Institute of Environment and Ecology, Jiangsu University, Zhenjiang 212013, China | kjj80116@163.com | | ZHANG Xu-yun | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | CHEN Bin | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | DU Dao-lin | College of Environmental and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
Institute of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China | daolindu@163.com |
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| Abstract: |
| A rapid and sensitive detection method was established in this study for evaluating the environmental behavior of tetrabromobisphenol A bis(2-hydroxyethyl)ether(TBBPA-DHEE). On the basis of its structure, hapten D3 was designed and synthesized, and five monoclonal antibodies against TBBPA-DHEE were prepared using hybridoma technology. These antibodies showed high cross-reactivity with tetrabromobisphenol A mono(2-hydroxyethyl)ether(TBBPA-MHEE)and no cross-reactivity with other TBBPAs. After the reaction conditions were optimized, the results showed that the linear ranges of TBBPA-MHEE and TBBPA-DHEE were 0.86~13.70 ng·mL-1 and 0.96~8.10 ng·mL-1 with detection limits of 0.78 ng·mL-1 and 0.56 ng·mL-1, respectively. Moreover, in water and soil samples collected near a brominated flame retardants(BFR)plant in Shouguang City, Shandong Province, the maximum concentrations of TBBPA-DHEE/TBBPA-MHEE were 15.45 ng·mL-1 and 6.75 ng·g-1, respectively. |
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