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| Diversity of antibiotic resistance genes in paddy soils in Sichuan Province, China |
| Received:December 09, 2019 |
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| KeyWord:paddy soils;antibiotic resistance genes;high throughput qPCR |
| Author Name | Affiliation | E-mail | | HE Yan | Key Laboratory of Southwest China Wildlife Resources Conservation/College of Life Sciences, China West Normal University, Nanchong 637002, China | | | ZHU Dong | College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China | | | WANG Dong | College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China | | | LIU Yan | College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China | | | PENG Chao | Key Laboratory of Southwest China Wildlife Resources Conservation/College of Life Sciences, China West Normal University, Nanchong 637002, China | | | HUANG Li-ping | Key Laboratory of Southwest China Wildlife Resources Conservation/College of Life Sciences, China West Normal University, Nanchong 637002, China | | | PENG Zheng-song | Key Laboratory of Southwest China Wildlife Resources Conservation/College of Life Sciences, China West Normal University, Nanchong 637002, China
College of Agriculture Science, Xichang College, Xichang 615000, China | | | LU Lu | College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China | llu327@cwnu.edu.cn |
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| Abstract: |
| To clarify the abundance and diversity of antibiotic resistance genes(ARGs)and their correlation with environmental factors in Sichuan Province, a high-throughput quantitative PCR technique(HT-qPCR)was performed on seven geographically separated paddy soils using 283 ARGs and 12 mobile genetic elements(MGEs)primers sets. The results showed that a total of 166 ARGs and 9 MGEs were detected in all seven paddy soils, with a range of 56~84 ARGs in each soil. The ARGs profiles in the seven paddy soils varied in their compositions and dominant ARGs types, and each soil contained their unique ARG sub-types. The relative abundance and absolute abundance of ARGs among paddy soils were different. The ARGs copy number ranged from 9.55×108 to 2.83×1010 copies·g-1(dry soil), and the relative gene copy number was 0.012±0.006 copies·cell-1. The abundance of ARGs was significantly correlated(P<0.01)with that of MGEs, which suggests that horizontal gene transfer could play a vital role in the propagation and enrichment ARGs in agricultural soils. The top three ARGs types with relatively higher abundances were multidrug resistant(49.26%), macrolides-lincosamides-streptavidin B (MLSB) (11.30%), and beta lactamase(10.87%). Redundant analysis showed that total nitrogen, an important indicator of soil fertility, was significantly correlated with ARGs profiles in the seven paddy soils(P<0.01). In summary, a regional difference in ARGs patterns was observed in the Sichuan province. The distinct distribution patterns of ARGs among different paddy soils may be influenced by farmland managements. The implication of this study is that it can be used to objectively assess agricultural environmental risks of ARGs. |
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