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| Effects of silver nanoparticle(AgNP)on soil nitrogen-fixing microbial community structure and nitrogenase activity |
| Received:July 18, 2019 |
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| KeyWord:silver nanoparticle (AgNP);nitrogen-fixing microorganisms;community structure;nitrogenase activity;soil |
| Author Name | Affiliation | E-mail | | WU Ling-li | Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | YANG Yu-rong | Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | ZHANG Li | Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | LIU Xiao-hong | Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | SI You-bin | Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | youbinsi@ahau.edu.cn |
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| Abstract: |
| To evaluate the possible risks of silver nanoparticles(AgNP)in soil environments, culture experiments were carried out to study the effects of 10 nm (10, 25, and 50 mg·kg-1)and 50 nm (25, 50, and 100 mg·kg-1)AgNP on nitrogenase activity and the quantity and community structure of nitrogen-fixing microorganisms. The results showed that the number of nitrogen-fixing microorganisms declined; the relative abundance of Bradyrhizobiu, Pseudomona, Vulgatibacter, and Anaeromyxobacter decreased. In contrast, the relative abundance of Azoarcus increased in the AgNP-treated soils. In addition, the community structure of nitrogen-fixing microorganisms changed significantly upon exposure to AgNP in soil. The largest impact of soil AgNP on the structure of the nitrogen-fixing microbial community was seen for 10 nm AgNP applied for 7 days, while the smallest effect was seen for 50 nm AgNP applied for 90 days. The number of Azotobacter decreased by 18.96%~47.28% and 17.42%~27.78% after 28 days of soil exposure with different doses of 10 nm and 50 nm AgNP, respectively. Nitrogenase activity also decreased with exposure time, and by 14.55%~27.47% and 17.87%~21.79% after 90 days of soil exposure with different doses of 10 nm and 50 nm AgNP, respectively. In conclusion, AgNP have negative effects on soil nitrogen fixing microorganisms and nitrogenase activity, which are related to the AgNP size, dose, and exposure time. |
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