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| Effect of microcystins on nutrient absorption of rice seedling |
| Received:April 18, 2019 |
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| KeyWord:microcystins;rice seedling;nutrient absorption;plasma membrane H+-ATPase activity;gene expression |
| Author Name | Affiliation | E-mail | | LIU Hong-yue | Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China
Jiangsu Cooperative Innovation Center of Technology and Material of Water Treatment, Wuxi 214122, China | | | SHEN Ze-hui | Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | LIANG Chan-juan | Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China
Jiangsu Cooperative Innovation Center of Technology and Material of Water Treatment, Wuxi 214122, China | liangchanjuan@jiangnan.edu.cn |
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| Abstract: |
| In order to further analyze the adaptation mechanism of crops to microcystins(MCs) in water, we studied the effects of MCs on nutrient absorption and the expression of the genes encoding plasma membrane H+-ATPase in rice seedlings, using a hydroponic experiment. After exposure to MCs for 7 days, the root activity(dehydrogenase activity) of rice seedlings treated with 1 μg·L-1 MCs increased. The root activity and the activity of plasma membrane H+-ATPase of roots of rice seedlings treated with 10 μg·L-1 MCs increased, promoting the absorption of mineral nutrients(Mg2+, Fe2+, Zn2+ and NO3-). The increase in plasma membrane H+-ATPase activity was related to the up-regulation in expression levels of genes OSA1, OSA2, OSA3, OSA4, OSA6, OSA8 and OSA9. High concentration of MCs(100 μg·L-1 and 1000 μg·L-1) reduced the root activity and plasma membrane H+-ATPase activity of rice seedlings, inhibiting the absorption of mineral nutrients. The expression levels of genes encoding plasma membrane H+-ATPase showed a trend of down-regulation and the degree of reduction of nutrient absorption increased with increasing concentration of MCs. After recovery for 7 days, root activity, plasma membrane H+-ATPase activity and mineral nutrient content in rice seedlings treated with 10 μg·L-1 MCs returned to control levels. All parameters in plants treated with 100 μg·L-1 MCs recovered compared to those measured during the exposure period. The inhibition of absorption of mineral nutrients caused by 1000 μg·L-1 MCs did not recover. In conclusion, the changes in nutrient absorption in rice seedlings caused by MCs are regulated by plasma membrane H+-ATPase activity, and the effect of regulation is dependent on the concentration of MCs. |
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