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| Optimization of the acidification condition in anaerobic fermentation of food waste and cow manure based on response surface methodology |
| Received:April 06, 2019 |
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| KeyWord:food waste;cow manure;anaerobic fermentation;response surface;acidification condition |
| Author Name | Affiliation | E-mail | | LIU Jin-li | Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-waste in Cold Region, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163411, China | | | HONG Xiu-jie | Daqing Agricultural Technology Promotion Center, Daqing 163411, China | | | BAI Yan | Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-waste in Cold Region, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163411, China | | | BI Shao-jie | Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-waste in Cold Region, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163411, China | | | YANG Hong-zhi | Food College of Heilongjiang Bayi Agricultural Reclamation University, Daqing 163319, China | | | WANG Yan-jie | Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-waste in Cold Region, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163411, China | wangyanjie1972@163.com |
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| Abstract: |
| The objective of the study was to create appropriate conditions for waste recycling anaerobic fermentation. Therefore, the optimization of acidification conditions during the mixed anaerobic fermentation of food waste and cow manure was conducted using response surface methodology. Single factor experiments showed the optimal stirring frequency, stirring rate, acidification concentration, acidification time, and acidification temperature were 3 times·d-1 (2 min·time-1), 50 r·min-1, 12%, 8 h, and 35℃, respectively. Based on this, the fermentation process in acidification conditions was optimized. The effect of the three factors, acidification temperature, acid concentration, and acidification time on the fermentation process was studied using methane production rate as the response value. The results of the BoxBehnken design and response surface methodology showed the optimum fermentation was at 7.6 h, acidification time; 10.4%, acidification concentration; and 35.8℃, acidification temperature. The optimal stirring frequency and speed were 3 times·d-1 (2 min·time-1) and 50 r·min-1, respectively. After the optimal acidification treatment, the methane production rate of the mixed raw material can reach 290.5 mL·g-1 VS, which is 17.9% higher than that of the raw material without the acidification treatment. Optimization of acidification conditions of the acidification phase in anaerobic fermentation of cow manure and kitchen waste can improve methane production rate, methane content, and volatile solid removal rate. |
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