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| Isolation and biological characterization of phosphorus accumulating organisms(PAOs)from greenhouse facility soil |
| Received:May 14, 2019 |
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| KeyWord:phosphorus accumulating organisms;phosphorus removal;biological characteristics;greenhouse facility;soil |
| Author Name | Affiliation | E-mail | | WU Xiao-qing | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | Lü Yu-ping | Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201600, China | | | ZHOU Fang-yuan | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | ZHAO Xiao-yan | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | ZHANG Guang-zhi | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | REN He | Shandong New Times Pharmaceutical Co., Ltd., Linyi 273400, China | | | ZHOU Hong-zi | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | WANG Jia-ning | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | | | ZHANG Xin-jian | Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China | zhangxj@sdas.org |
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| Abstract: |
| The objective of this study was to isolate efficient phosphorus accumulating organisms (PAOs) with good environment adaptability. Soil samples (0~40 cm depth) were collected from within greenhouses. The PAOs were isolated and screened by serial dilution, PAOplate culturing, and metachromatic granule staining. Taxonomic identification of PAO isolates was based on morphological characters and 16S rRNA sequencing. Phosphorus removal and phosphorus contents of these PAOs were determined by the Mo-Sb colorimetric method. Gene ppk, the key gene in phosphorus-accumulation, was amplified using PCR. Salt and high temperature tolerances were analyzed by counting the number of surviving bacteria following exposure. Antagonistic activity against pathogens was determined by a confrontation test.Seven bacterial PAOs belonging to 4 genera, Arthrobacter, Microbacterium, Rhodococcus, and Bacillus were screened.All strains showed phosphorus-removal effects, of which A. ureafaciens Fp64 showed the largest removal effect (70.52%), and R.rhodochrous Fp31 had the highest phosphorus content (18.74%). The ppk gene was detected in 4 strains with phosphorus removal rate >50%. The PAO strains also showed variable biological characters. A. ureafaciens Fp64 and A.keyseri Fp38 were salt tolerant and antagonistic to pathogens. M.esteraromaticum Np20 showed antagonistic activities and high temperature tolerance. R.rhodochrous Fp31 exhibited prominent salt tolerance. The PAO strains screened in this study had high phosphorus contents and phosphorus removal capacities. Furthermore, they exhibited variable features, including antagonistic activity and environmental adaptability. They are expected to be used to reduce phosphorus loss from soil leaching and act as good agents for biological phosphorus removal technology in the greenhouse industry. |
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