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| Study on the damage caused by dimethyl phthalate on Pseudomonas fluorescens |
| Received:November 30, 2018 |
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| KeyWord:Dimethyl phthalate;Pseudomonas fluorescens;membrane damage;leakage of dissoluble matter |
| Author Name | Affiliation | E-mail | | GUO Ru-xin | College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006, China | | | WANG Zhi-gang | College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006, China | wzg1980830@sina.com | | WANG Chun-long | College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006, China | | | YOU Yi-min | College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China | | | WANG Heng-xu | College of Life Science and Agroforestry, Qiqihar University, Qiqihar 161006, China | |
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| Abstract: |
| Dimethyl phthalate(DMP)is a ubiquitous pollutant that is very harmful to microorganisms. Pseudomonas fluorescens(P. fluorescens)is one of the most important beneficial bacteria in the environment. To explore the toxicological effects of DMP on bacteria, P. fluorescens was selected and investigated using several bio-techniques, such as the polarization method, transmission electron microscopy, and ultra-high performance liquid chromatography. The results showed that the specific growth rate of P. fluorescens was slower, but the generation time was extended under the 10, 20 mg·L-1, and 40 mg·L-1 DMP treatments. The zeta potential on the surface of P. fluorescens decreased gradually from -12.21±0.56 mV to -18.13±0.67 mV under the 0, 10, 20 mg·L-1, and 40 mg·L-1 DMP treatments. Meanwhile, the mobility of the cell membrane increased by the DMP treatment and resulted in obvious cell deformation, severe plasmolysis, and leakage of dissoluble matter. The cumulative concentration of DMP inside the cells increased from 0.11±0.10 mg·L-1 to 6.78±0.25 mg·L-1, and the cumulative concentration outside the cells increased from 8.44±0.37 mg·L-1 to 30.52±0.88 mg·L-1, under the 10, 20 mg·L-1, and 40 mg·L-1 DMP treatments. Therefore, we conclude that DMP destroys the cell wall and the cell membrane of P. fluorescens and strongly affects the normal growth of P. fluorescens. |
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