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| Effects of amendments with different C/N/P ratios on the microbial community structure in Pb-Zn mine tailings |
| Received:December 16, 2018 |
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| KeyWord:Pb-Zn mine tailing;amendments with different C/N/P ratios;microbial community;microbial diversity;microbial activity;microbial biomass |
| Author Name | Affiliation | | YANG Sheng-xiang | College of Resources and Environment and Key Laboratory of Soil Resources and Environment in Qianbei of Guizhou Province, Zunyi Normal University, Zunyi 563006, China
College of Biology and Environmental Science, Jishou University, Jishou 416000, China | | LI Feng-mei | College of Biology and Environmental Science, Jishou University, Jishou 416000, China | | PENG Xi-zhu | College of Biology and Environmental Science, Jishou University, Jishou 416000, China | | CAO Jian-bing | Xiangxi Environmental Protection Bureau, Xiangxi 416000, China | | GAO Zhi-xi | College of Resources and Environment and Key Laboratory of Soil Resources and Environment in Qianbei of Guizhou Province, Zunyi Normal University, Zunyi 563006, China |
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| Abstract: |
| As an important component of the soil ecosystem, soil microorganisms play a key role in the ecological restoration of mining wastelands. Analyses of the soil microbial communities in these areas will provide valuable information for understanding the restoration processes of these degraded ecosystems. A field test was carried out to restore the ecosystem using amendments with different C/N/P ratios and subsequently evaluate the effects of these amendments on the soil microbial community in an abandoned Pb-Zn mine tailings. We obtained the following results:Compared to the control, the use of amendments with different C/N/P ratios significantly increased the operational taxonomic units (OTUs), microbial diversity (measured using the Chao1, ACE, and Simpson's and Shannon's indices), microbial activity and microbial biomass. The soil microbial community structure changed significantly when amendments with different C/N/P ratios were used, in comparison to the control. Among the top 10 dominant phyla, the relative abundance of the phyla Bacteroidetes, Euryarchaeota, Verrucomicrobia, Acidobacteria and Candidate division OD1 increased, and the relative abundance of the phylum Cyanobacteria decreased. Similarly, among the top 10 abundant genera, the relative abundance of the genera Solitalea, Sphingomonas, Blastocatella, Planctomyces, Opitutus, Ohtaekwangia and Gemmata increased, and the relative abundance of the genus Gaiella decreased. An analysis of the Pearson's correlation coefficients showed that, at the phylum level, the phyla Bacteroidetes, Planctomycetes, Euryarchaeota and Verrucomicrobia were positively correlated with microbial activity and biomass, while the phyla Chloroflexi and Cyanobacteria were negatively correlated with microbial activity and biomass. At the genus level, a significant positive correlation was observed between the genera Solitalea, Opitutus, Blastocatella and Ohtaekwangia and microbial activity and biomass, while a significant negative correlation was observed between the genus Gaiella and microbial activity and biomass. An aggregated boosted tree (ABT) analysis revealed that, at the phylum level, the CK, N and MHR+N samples were clustered together, while the P, MHR, MHR+P and MHR+N+P samples formed a close cluster. At the genus level, the N, P, MHR, MHR+N, MHR+P and MHR+N+P samples were clustered together, while CK formed a separate cluster. In conclusion, all the results indicate that amendments with different C/N/P ratios significantly affect the soil microbial community structure, diversity, activity and biomass, and the amendments MHR+P and MHR+N+P are the most effective. |
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