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| Responses of archaeal community composition in paddy soils to straw return |
| Received:April 17, 2018 Revised:June 07, 2018 |
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| KeyWord:archaeal community;straw return;community response;influencing factors |
| Author Name | Affiliation | E-mail | | WANG Ning | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | | | ZHAO Ya-hui | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | | | AI Yu-chun | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | | | ZHANG Yong-chun | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | | | WANG Ji-dong | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | | | YU Jian-guang | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Scientific Observing and Experimental Station for Farmland Conservation(Jiangsu), Ministry of Agriculture, Nanjing 210014, China | yujg@jaas.ac.cn |
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| Abstract: |
| To explore the impacts of straw return on the composition of archaeal communities in paddy soils, different amounts of wheat straw (0, 1%, 2%, and 5%)were incorporated into two types of paddy soils (silty and clayed soil), which were incubated with flooding in the lab over a 60 d period. At time intervals of 0, 15, 30, and 60 d, soil samples were collected and analyzed for soil physico-chemical properties and archaeal communities based on high-throughput sequencing. Results showed that straw addition significantly decreased soil pH while increasing soil electricity conductivity (EC), total carbon and nitrogen (TC and TN)contents, and C/N ratio and concentrations of dissolve organic carbon (DOC), ferrous[Fe (Ⅱ)], and total ferric (Fe)in soil porewater (P<0.05). Higher application rates of straw lead to greater changes in the soil properties. Based on illumina sequencing technology, the major component of the archaeal community was Thaumarchaeota at the phylum level for both soils with and without wheat straw. During the incubation period of 0~30 d, straw return had insignificant influences on the relative abundance of Thaumarchaeota for both soils, which was maintained at 97%~98%. However, at the time interval of 60 d, straw addition at rates of 1%, 2%, and 5% significantly reduced the relative abundances of Thaumarchaeota in both soils. Principle component analysis (PCA)based on archaeal abundance at the species level showed that there were significant (P<0.05)differences in archaeal community composition between the two soil types, indicating soil-dependent archaeal community composition. Compared to that in unamended soils, significant shifts in archaeal community composition were observed in the silty soil when amended with 5% straw, and in the clayed soil when amended with 1% straw. Further analyses showed significant (P<0.05)differences in archaeal community compositions of the silty soil between low (1% and 2%)and high (5%)rates of straw incorporation, whereas there were insignificant differences between 1% and 2% straw treatments. Unlike the silty soil, significant differences in archaeal community compositions were observed for the clayed soils when amended with 1% and 2% of straw. Using an envfit function (999 permutations), soil pH, EC, C/N ratio, DOC, Fe (Ⅱ), and Fe that significantly correlated with the archaeal community composition at the species level were selected in an RDA analysis, revealing that pH and C/N ratio were the dominant variables that significantly contributed to the variance of archaeal communities in both soils. In conclusion, straw addition significantly changed archaeal community composition by altering soil chemical characteristics, and the response of soil archaeal community composition to straw return depended on soil type. |
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