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| Impacts of ciprofloxacin in duck feces on antibiotic resistance genes in ustic cambosols |
| Received:March 26, 2018 |
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| KeyWord:duck feces;ciprofloxacin;ustic cambosols;antibiotic resistance genes;mobile genetic elements;soil physicochemical properties |
| Author Name | Affiliation | E-mail | | CHANG Xu-hui | College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China | | | JIA Shu-gang | Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Guangxi Teachers Education University, Nanning 530001, China | | | WANG Shu-ping | College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China | wshuping@ucas.ac.cn | | ZHOU Zhi-qiang | College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China | |
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| Abstract: |
| In order to explore the impacts of ciprofloxacin in duck feces on antibiotic resistance genes (ARGs) in ustic cambosols, an 81-d soil culture experiment was conducted with five treatments:CK (control), CIP (ciprofloxacin), DF (duck feces), DF + CIP (DF with added CIP), and DF (CIP) (DF containing CIP). Twenty-seven kinds of ARGs and four kinds of mobile genetic elements (MGEs) were detected by polymerase chain reaction (PCR) testing. Absolute abundance of target genes with relatively high detection rates and total bacterial gene (16S rRNA) were determined by quantitative real time PCR testing. The results showed that six ARGs (tetG, sulⅠ, qnrA, qnrS, aadA2, and aadD) and one MEG (intⅠ) were found in different treatments, and these genes were essentially identical. The effects of DF (CIP) and DF + CIP on ARGs and bacteria in soil were different. Compared with DF, DF (CIP) and DF + CIP significantly reduced the absolute abundance of tetG and 16S rRNA. DF (CIP) enhanced the absolute abundance of sulⅠ and aadA2, and DF + CIP enhanced the absolute abundance of qnrA. Partial correlation analysis among ARGs, MGEs and soil physicochemical properties, showed that intⅠ was significantly positive correlated with sulⅠ and aadA2, but was significantly negatively correlated with qnrA. The positive correlation was observed between qnrA and residual contents of ciprofloxacin, and between tetG and soil organic matter. The results of this study provide a theoretical basis for scientific assessment of environmental risks posed by fluoroquinolones and suitable application of manure. |
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