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| Activity and abundance of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of marsh wetland soil in Northeast of China |
| Received:July 15, 2017 |
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| KeyWord:ammonia-oxidizing bacteria(AOB);ammonia-oxidizing archaea(AOA);potential ammonia oxidation activity(PAO);the abundance of AOA/AOB;environmental factors |
| Author Name | Affiliation | E-mail | | XIE Yue | Harbin Institute of Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin 150090, China | | | LIANG Hong | Forestry College of Northeast Forestry University, Harbin 150040, China | hongliang@nefu.edu.cn | | SONG Li-quan | Forestry College of Northeast Forestry University, Harbin 150040, China | | | WANG Qing-bo | Heilongjiang Sanjiang National Nature Reserve Administration, Fuyuan 156500, China | | | FU Dong-feng | Heilongjiang Sanjiang National Nature Reserve Administration, Fuyuan 156500, China | | | GAO Da-wen | Harbin Institute of Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin 150090, China
Forestry College of Northeast Forestry University, Harbin 150040, China | gaodw@hit.edu.cn |
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| Abstract: |
| In this study, chlorate and ampicillin were used as inhibitors to nitrite-oxidizing bacteria(NOB) and ammonia-oxidizing bacteria(AOB), respectively. And combined with the quantitative polymerase chain reaction(qPCR) to test two different properties of typical marsh wetland topsoil in Northeast China, Sanjiang plain and Zhalong plain. The correlation of activity and abundance of AOA and AOB in growing season and non-growing season was analyzed. The results showed that the pH value could significantly affect the activity of AOA(P<0.01) in different types of wetland soils; The abundance value of AOA and PAO（AOA+AOB） had significantly positive correlation(r=0.96, P<0.05), in turn, with its PAO（AOA） had no correlation(P>0.5). The PAO（AOA+AOB） and PAO（AOA）, the abundance of AOA and AOB of Sanjiang wetland had no significant difference between Calamagrostis angustifolia and Carex lasiocarpa, indicting the difference of plants would not affect the PAO and the abundance of ammonia-oxidizing microbial. However, after inhibited the activity of AOB, the PAO value emerged significantly decreased(P<0.05), which concluded AOB could play an important role in the ammonia oxidation process of Carex lasiocarpa. |
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