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| Degradation of pyrene by immobilized cells of fusant bacterial strain F14 using biochar |
| Received:September 29, 2017 |
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| KeyWord:fusant bacterial strain;immobilized microorganism;biochar;PAHs |
| Author Name | Affiliation | E-mail | | ZHANG Song | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | | | HOU Bin | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | | | JI Ting-ting | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | | | LIU Yang | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | | | HUANG Fei | The College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | | | QIN Zi-fei | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | | | LU Jing | School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China | lujing19822@163.com |
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| Abstract: |
| F14 is a fusant strain constructed by protoplast fusion and is able to degrade polycyclic aromatic hydrocarbons(PAHs). To improve the pyrene removal rate, F14 was immobilized using biochar. The removal of pyrene by immobilized beads with added biochar produced at different temperatures(300, 500℃, and 700℃) was investigated, based on a study of the optimum combination ratio of polyvinyl alcohol(PVA) and sodium alginate(SA). The results showed that the diameter of the immobilized beads was about 3 mm, and an analysis of the effects, mass transfer, and mechanical properties of the immobilized beads showed that the best ratios of PVA and SA were 8% and 1%, respectively. Further analysis of mass transfer and scanning electron microscopy of the immobilized beads made by different biochars showed that the immobilized beads made from 700℃ biochar had better performance. Immobilized cells presented a higher and faster removal ability than free cells for pyrene, and 94.91% of pyrene could be removed within 10 d from an initial concentration of 50 mg·L-1. The percentage removed by the immobilized cells was 58% higher than that removed by free cells. In addition, the immobilized beads could be reused up to 9 times. |
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