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| Isolation and degradation characteristics of hydrocarbon-degrading endophytic bacteria from the salt-tolerant plant Chloris virgata |
| Received:May 31, 2017 |
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| KeyWord:petroleum contamination;saline soil;endophytic bacteria;Bacillus pumilus;salt-tolerant plant;petroleum degradation |
| Author Name | Affiliation | | WU Tao | Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, School of Bioengineering, Binzhou University, Binzhou 256600, China
Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou 256600, China | | XU Jie | Department of Bioengineering, Binzhou Vocational College, Binzhou 256603, China | | XIE Wen-jun | Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, School of Bioengineering, Binzhou University, Binzhou 256600, China
Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou 256600, China | | YAO Zhi-gang | Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, School of Bioengineering, Binzhou University, Binzhou 256600, China | | LIU Jun-hua | Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou 256600, China | | YANG Hong-jun | Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou 256600, China | | SUN Chun-long | Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, School of Bioengineering, Binzhou University, Binzhou 256600, China
Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Binzhou 256600, China | | WANG Shu-ping | Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, School of Bioengineering, Binzhou University, Binzhou 256600, China |
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| Abstract: |
| The degradation characteristics of hydrocarbon-degrading endophytic bacteria isolated from salt-tolerant plants may provide the foundation for phytoremediation of petroleum-polluted saline soils using endophytic bacteria. Twenty-four strains of endophytic petroleum-degrading bacteria were isolated from the salt-tolerant plant Chloris virgata, which grows in a heavily saline-alkaline, crude oil-contaminated environment in the Yellow River Delta, China. All 24 strains were able to utilize diesel oil as their sole carbon and energy source. Their abilities to degrade diesel oil were compared to screen one highly effective petroleum-degrading bacterium named BF04. The strain BF04 was identified as Bacillus pumilus based on physiological and biochemical characteristics, and 16S ribosomal DNA analysis. In addition, we studied the kinetic characteristics of diesel oil degradation and the capability of the strain to utilize different hydrocarbons. The results showed that strain BF04 could utilize straight-chain paraffin, branched paraffin, and mononuclear aromatics, but could not utilize phenol or polycyclic aromatic hydrocarbons. The degradation rate of diesel-oil short-chain n-alkanes(nC11~nC23) was relatively higher than that of long-chain n-alkanes(nC24~nC27) in the mineral salts(liquid) medium. The degradation of petroleum may be expressed using a first-order kinetic model. Moreover, the strain BF04 degraded diesel-oil effectively in the presence of 3% NaCl, indicating that strain BF04 has potential application in the bioremediation of petroleum-contaminated saline-alkaline soils. |
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