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| Characteristics of DNA adsorption and desorption on micro-interfaces of black soil colloids |
| Received:April 27, 2017 |
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| KeyWord:black soil colloid;DNA;coarse organic colloid;coarse inorganic colloid;adsorption isotherms;desorption |
| Author Name | Affiliation | | WANG Zhi-gang | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | CHEN Wen-jing | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | HU Ying | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | XU Wei-hui | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | WANG Chun-long | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | LÜ Zhi-hang | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China | | LIU Ze-ping | Institute of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China |
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| Abstract: |
| The characteristics of DNA adsorption and desorption affect the presence of intact DNA and are of great significance for genetic transformation on micro-interfaces of soil colloids. The adsorption and desorption characteristics of DNA by coarse organic colloid(COC) and coarse inorganic colloid(CIC) were studied using the balanced reaction method. The results showed that the rate and quantity of DNA adsorption were lesser on CIC than on COC. The quantity of the adsorbed DNA on black soil colloids decreased gradually with increasing pH. Langmuir adsorption equation could describe the isothermal adsorption of DNA by COC and CIC well, with corresponding determination coefficients(R2) of 0.972 and 0.991, respectively. The equilibrium constant of Langmuir(K) of COC was higher than that of CIC, which proved that the adsorption affinity of COC was better than that of CIC. The rates of DNA desorption from COC and CIC were 63.5% and 47.4%, respectively. The present study indicates that DNA can be adsorbed onto the surface of black colloid, the organic matter contributed greatly to the capability of the DNA adsorption, and that pH is an important factor affecting the adsorption of DNA on black soil colloids. |
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