|
| Biomineralization of Pb by a strain of Achromobacter xylosoxidans and its practical application in bioremediation |
| Received:March 24, 2017 |
| View Full Text View/Add Comment Download reader |
| KeyWord:Achromobacter xylosoxidans strain LAX2;biomineralization;available lead;bioremediation |
| Author Name | Affiliation | E-mail | | LI Zhe | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | CHEN Tong-yue | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | LENG Su | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | WU Di | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | ZHANG Xiu-fang | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | ZHOU Ye | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | CHE Chi | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | | | LI Ming-tang | College of Resource and Environmental Science, Key Laboratory of Soil Resource Sustainable Utilization for Jilin Province Commodity Grain Bases, Jilin Agricultural University, Changchun 130118, China | limtdoc2008@163.com |
|
| Hits: 2584 |
| Download times: 3411 |
| Abstract: |
| The biomineralization of Pb by a fermentation broth of bacterial strain Achromobacter xylosoxidans LAX2 growing in a urea-containing medium, and the effect of Pb immobilization effect in soil, were studied. The results showed that the growth of LAX2 was not significantly affected when the concentration of Pb in the medium was less than 105 mg·L-1. Scanning electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and energy spectrum analysis showed that the fermentation broth produced by the LAX2 growing in a urea-containing culture system formed a spherical PbCO3 crystal, with a maximum diameter of 2 μm, by biomineralization. The Pb2+ removal rates via biomineralization, bacterial cell adsorption, and chemical precipitation were 86.3%, 91.6%, and 67.4% respectively, indicating that there were different mechanisms in the three processes. Two days after incubation with the LAX2 fermentation broth, the amount of available Pb in the soil decreased rapidly; but after 5 d, the tendency slowed down. Overall, the amount of available Pb in the soil showed a declining trend during 30 d of incubation. The content of available Pb in the soil decreased 56.1%,76.5%, and 86.8% after 5, 10, and 30 d of incubation, respectively. Compared with the control groups, the addition of fermentation broth, sterile fermentation broth, and bacterial cells to Pb-contaminated soil increased the heights of Chinese cabbage plants by 49.4%, 21.6%, and 9.6%, respectively;the content of available Pb in the soil decreased by 78.1%, 64.2%, and 52.4%, respectively; and the content of Pb in the Chinese cabbage decreased by 56.5%, 27.4%, and 14.5%, respectively. These results indicate that the strain LAX2 has important value in bioremediation of Pb-contaminated soil, due to its ability to form crystalline PbCO3 with via biomineralization, leading to Pb immobilization and a reduction of Pb toxicity to, and accumulation in plants. |
|
|
|
