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| Interaction properties of buprofezin to calf thymus DNA in the presence of metal ions |
| Received:May 30, 2016 |
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| KeyWord:buprofezin;metal ions;ctDNA;fluorescence spectrum |
| Author Name | Affiliation | E-mail | | ZHU Na | Environmental and Resource College, Shanxi University, Taiyuan 030006, China | | | HE Ya-mei | Environmental and Resource College, Shanxi University, Taiyuan 030006, China | | | LIANG Dong | School of Chemical and Environmental Engineering, North University of China, Taiyuan 030051, China | | | SANG Nan | Environmental and Resource College, Shanxi University, Taiyuan 030006, China | sangnan@sxu.edu.cn |
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| Abstract: |
| To illustrate the genetic toxicity mechanism of thiadiazine pesticides on non-target organisms, the interaction between buprofezin and calf thymus DNA(ctDNA) in vitro was investigated by UV-vis absorption spectroscopy and fluorescent spectrometry. The influences of heavy metals(Cd2+, Co2+, Cu2+ and Ni2+) on the binding properties were also studied at the molecular level, using methylene blue(MB) as a probe of DNA. The results showed that there was a distinct red shift of absorption spectrum by addition of ctDNA which can be ascribed to the binding interaction between buprofezin and ctDNA. Fluorescent experiments indicated that buprofezin was bound to ctDNA in competition with MB, and the binding constants decreased as a function of temperature. Furthermore, DNA melting-point increment suggested that the binding of buprofezin to ctDNA was an intercalative mode. The presence of metal ions did not change the interaction mode between buprofezin and ctDNA, but influenced the binding intensity of buprofezin to ctDNA. A small quantity of Cu2+ and Ni2+ showed a promotion between buprofezin and ctDNA via an ionic-bridge mechanism, but a large quantity of ions would modify the structure of ctDNA and weaken the binding capacity. Meanwhile, Co2+ and Cd2+ could penetrate the ctDNA and competitively bind to ctDNA, resulting in the declination of binding constant. |
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