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| Impacts of domestic sewage on community structure and diversity of bacteria in the soil and rhizospheres of five wetland plants |
| Received:April 13, 2016 |
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| KeyWord:wetland plants, PCR-DGGE, edaphon, diversity |
| Author Name | Affiliation | E-mail | | LI Long-shan | Ningxia Forestry Institute Key Laboratory of the Seedling Bioengineering, Yinchuan 750004, China
Ningxia Ding-Shi Bioassay Center, Yinchuan 750002, China | | | NI Xi-lu | Ningxia Forestry Institute Key Laboratory of the Seedling Bioengineering, Yinchuan 750004, China
Yinchuan Urban Forest Ecosystem Research Station of State Forestry Administration, Yinchuan 750004, China | nixilu110@163.com | | LI Chang-xiao | Ningxia Forestry Institute Key Laboratory of the Seedling Bioengineering, Yinchuan 750004, China | | | LI Jian | Ningxia Forestry Institute Key Laboratory of the Seedling Bioengineering, Yinchuan 750004, China | |
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| Abstract: |
| The structure and diversity of bacterial communities were studied within the soil and within the rhizospheres of five wetland plants treated with tap water and sewage water using the PCR-DGGE technique. Results indicate that the dominant bacterial communities undergo a shift both within bare soil, and within the wetland plant root zone with the addition of sewage water, The bacterial community diversity index was highest within the rhizospheres of wetland plants that were treated with sewage water, followed by the soil without plants that was treated with sewage water, and then within the root zone of wetland plants immersed in tap water. The bacterial community diversity index was lowest in the un-amended experimental soil used for plant culture. The dominant phyla of the bacterial communities were analyzed by denaturing gradient gel electrophoresis(DGGE) and sequencing of partial 16S rRNA genes. There were eight genera of bacteria detected in our study overall. Within the un-amended experimental soil treatment, Rhizobium and Sphingomonas were the dominant genera; in the treatment with just soil and sewage, Sphingomonas, Rhizobium, Arthrobacter and Erythrobacter were the dominant genera; within the root zones of the wetland plants in tap water, Flavobacterium and Arthrobacter were the dominant genera; and within the root zones of the wetland plants in sewage water, Arthrobacter, Clostridium, Sulfate-reducing bacteria, Proteiniclasticum and Flavobacterium were dominant. This study provides evidence that sewage may promote the growth of diverse microbial communities in soil and within the rhizospheres of wetland plants. |
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