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| Performance of immobilized denitrifying bacteria in constructed wetland for slightly-polluted water treatment |
| Received:April 19, 2016 |
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| KeyWord:immobilization, constructed wetland, denitrification, microbial diversity |
| Author Name | Affiliation | E-mail | | LIN Yan | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | ZHANG Huan-jie | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | LIU Xi | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | YU Lu | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | ZHU Wen-ying | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | KONG Hai-nan | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | hnkong@sjtu.edu.cn |
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| Abstract: |
| Denitrifying bacteria Pseudomonas stutzeri isolated from lab were fixed with PVA and SA and were added to dosing positions(upper, lower, upper and lower) to simulate the release of the nitrate-rich source in low pollution water, aiming to determine the optimal dosing position.The reasons of enhanced nitrogen removal in microbial community diversity were analyzed by using high-throughput sequencing technology, and the effect of strengthening efficiency at low temperature was investigated. Results showed that the system dosing in upper layer got the best performance, and the removal rates of total nitrogen and nitrate were 60.31% and 64.98%, respectively. The results of microbial diversity index of each system showed that the microbial species diversity in the control system was higher than othes. But overall, there was little difference between the four systems, and the most abundant phylum was Proteobacteria in each system. And the increased percentage of Nitrospirain dosing systems was conducive to improve the nitrogen removal. The results showed that the removal rates of total nitrogen and nitrate in the system dosing in upper layer were 50.86% and 55.06%, respectively, while that in the control system were 24.81% and 27.53% at 15℃. |
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