|
| Isolation and identification of a cellulolytic bacterium and its composting application |
| Received:July 18, 2015 |
| View Full Text View/Add Comment Download reader |
| KeyWord:cellulose decomposing bacterium;screening and identification;optimized conditions;composting |
| Author Name | Affiliation | E-mail | | ZHANG Xi-qing | College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China | | | GOU Chang-long | College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China | | | LOU Yu-jie | College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Key Laboratory of Animal Production, Product Quality and Security, Jilin Agriculture University, Changchun 130118, China | | | XU Jia-ping | Institute of Special Animal and Plant Sciences of CAAS, Changchun 130112, China | | | GAO Yun-hang | College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China | gaoyunhang@163.com |
|
| Hits: 3126 |
| Download times: 3499 |
| Abstract: |
| Composting is a good approach to utilizing agricultural organic wastes. Supplying microorganisms could shorten composting period and improve compost quality. In this study, a cellulose-decomposing strain was isolated from natural composting manure by using congo red staining and CMC-Na plate method. The strain was identified according to morphological observation, biochemical testing and 16S rDNA gene sequence. The optimal fermentation conditions were also determined by using single factor and orthogonal experiment. Finally, the isolate was inoculated into cattle mature to test its effect. The strain Y2 was identified as Bacillus subtilis; Its FPA and CMCA were 15.83 U·mL-1 and 100.81 U·mL-1, 1.3 times and 2.76 times higher than originals, respectively. Compost inoculated with Y2 and EM microbial agents reached high temperature after 3 d, with high temperature maintained for 9 d and 8 d, respectively. The degradation rate of cellulose was 42% for Y2 group, while it was 35% for EM microbial agent and 10.5% for sterile water. Therefore, strain Y2 has a great potential to be applied to composting fermentation. |
|
|
|
