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Effects of cellulose-decomposing microbes and sawdust on cellulose enzyme activity and odor during animal waste degradation |
Received:July 23, 2015 |
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KeyWord:cellulose microbial community;sawdust;animal waste |
Author Name | Affiliation | E-mail | HUANG Wang-zhou | College of Animal Science and Technology of Gansu Agricultural University, Lanzhou 730070, China | | ZHANG Sheng-wei | College of Animal Science and Technology of Gansu Agricultural University, Lanzhou 730070, China | | GUN Shuang-bao | College of Animal Science and Technology of Gansu Agricultural University, Lanzhou 730070, China | gunsb@gsau.edu.cn | YAO Tuo | Pratacultural College of Gansu Agricultural University, Lanzhou 730070, China | | ZHU Jian-xun | College of Animal Science and Technology of Gansu Agricultural University, Lanzhou 730070, China | |
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Abstract: |
Nowadays, disposing and utilizing recalcitrant materials in animal waste have drawn wide attention because of low resource utilization and serious environmental pollution in China. Recent research has showed that adding microbial inocula and bulking agents can improve utilization of animal wastes and maximize resource recycling. In this study, effects of cellulose-decomposing microbes on animal wastes was studied by using “2×4 experimental design” with sawdust and microbe additions as two factors, and animal waste alone as a control. Carboxymethyl cellulose(CMC) activity and NH3 and H2S releases were measured by DNS, boric acid, and zinc amine complex salt absorption methods, respectively. Results showed that CMC enzyme activity was reduced by sawdust alone, but was increased by the treatment with 5% cellulose-decomposing bacteria and 10% sawdust, with the maximum CMC enzyme activity of 2.54 mg·mL-1 and 2.33 mg·mL-1 for pig manure and cow manure, respectively. Amount of NH3 and H2S releases was decreased. |
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