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| Oxidative Stress and DNA Damage in Zebrafish Embryos Exposed to BDE-47 |
| Received:June 24, 2015 |
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| KeyWord:BDE-47;zebrafish embryos;oxidative stress;DNA damage |
| Author Name | Affiliation | E-mail | | ZHAO Xue-song | College of Environmental Science and Engineering, Jilin Normal University, Siping 136000, China | | | REN Xin | College of Environmental Science and Engineering, Jilin Normal University, Siping 136000, China | | | YANG Chun-wei | College of Environmental Science and Engineering, Jilin Normal University, Siping 136000, China | | | YOU Hong | Harbin Institute of Technology, Harbin 150090, China | | | REN Bai-xiang | College of Environmental Science and Engineering, Jilin Normal University, Siping 136000, China | renbaixiang008@126.com |
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| Abstract: |
| Polybrominated diphenyl ethers(PBDEs), widely used flame retardants, have been detected in environment, biota, and even human blood serum. Currently, little is known about their toxicity to fish. This experiment was designed to explore the oxidative stress and DNA damage of 2,2',4,4'-tetrabromodi-phenyl ether(BDE-47) to zebrafish embryos. Superoxide dismutase(SOD), catalase(CAT), total glutathione(GSH), malondialdehyde(MDA), and relative mRNA expression levels of genes(Sod1、Ucp-2、Gstp2 and Nqo1) were assessed in zebrafish embryos exposed to 1, 5, 10, and 50 μg·L-1 of BDE-47 for 96 hours. The single cell gel electrophoresis was performed to observe the DNA damage caused by BDE-47. Compared with control, reactive oxygen species(ROS) and MDA were increased by BDE-47 treatments in a dose-effect manner. Exposure to 50 μg·L-1 of BDE-47 for 96 hours significantly increased the activities of SOD and CAT, but significantly decreased GSH levels. Moreover, the transcription of Ucp-2 was significantly up-regulated to 4.12 folds(p<0.01) while that of Gstp2 and Nqo1 was significantly down-regulated to 0.62 folds and 0.55 folds(p<0.01), respectively, by 50 μg·L-1 BDE-47. There was no significant difference observed in the expressions of Sod1 between the treatments and the control. Under exposure of 5, 10, and 50 μg·L-1, olive tail moment increased significantly with increasing concentrations of ROS. These results indicate that BDE-47 could induce oxidative stress in zebrafish embryos and ROS is the trigger inducing embryos DNA damage. |
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