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| Oxidative Damages of Proteins in Hepatopancreas of Procambarus clarkii Exposed to Cu2+ |
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| KeyWord:Cu2+; Procambarus clarkii; hepatopancreas; protein oxidative damage |
| Author Name | Affiliation | | WEI Ke-qiang | School of Life Science, Shanxi University, Taiyuan 030006, China | | PANG Sheng-xi | Shanxi Center of Land Consolidation and Rehabilitation, Taiyuan 030024, China | | ZHAO Hui | School of Life Science, Shanxi University, Taiyuan 030006, China | | SONG Xin | Zhuozhou Branch School of Baoding Teachers College, Zhuozhou 072750, China |
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| Abstract: |
| Crayfish Procambarus clarkii is an ideal indicator of freshwater pollution. An experiment was designed to study the effects of Cu2+ on oxidative damages of proteins in hepatopancreas of Procambarus clarkii. The crayfishes were exposed to various concentrations of Cu2+（0, 0.5, 1.0, 3.0, 5.0 and 10.0 mg·L-1） for 96 h. Reactive oxygen species（ROS）, total antioxidant capacity（T-AOC）, glutathione peroxidase（GSH-PX）, protein carbonyl（PCO） content, and DNA-protein crosslinking（DPC） in the hepatopancreas tissues were determined at 24, 48, 72 and 96 h, respectively. ROS contents, T-AOC activity and DPC rates increased at lower, but decreased at higher concentrations of Cu2+ after exposure for 48, 72 and 96 h. PCO contents increased with Cu2+ concentrations and showed a dose-effect relationship. With Cu2+ concentration increasing, GSH-PX activity had “rise and decline” pattern at 24 h, while all decreased at 96 h. These results suggest that Cu2+ oxidative damages of proteins in the hepatopancreas may be one mechanisms of copper toxicity to Procambarus clarkii. |
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