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Genome-wide Transcriptional Responses in Rice Roots to the Co-contamination of Cadmium and Bensulfuron-methyl by Microarray
  
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KeyWord:Oryza sativa L.; co-contamination; gene expression; microarray; bioinformatics
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Abstract:
      Using the Affymetrix rice genome chip, it was explored that the gene transcriptional responses in YueXiangzhan and FengMeizhan rice roots to critical concentrations of cadmium(Cd, 45 μmol·L-1), bensulfuron-methyl(BSM, 0.25 μmol·L-1) and their co-contamination (++, 45 μmol·L-1 Cd +0.25 μmol·L-1 BSM) respectively. The results of microarray analysis showed that 2144 genes were significantly up-regulated(ratio≥2), and 2346 genes were down-regulated(ratio≤0.5) in the roots of the rice varieties treated with Cd, BSM and ++. Those highly up-regulated genes were classified into several groups, including sulfur assimilation, detoxification, anti-oxidation, protein ubiquitination and chaperones and so on. It revealed the mechanism for which the treatment of Cd combined with BSM alleviated the toxic effects of Cd or BSM individual treatment on FengMeizhan seedlings.