| 刘佳琪,张艺,孙耿,聂三安.干湿交替作用下水稻土DNRA菌的响应特征[J].农业环境科学学报,2025,44(8):2169-2177. |
| 干湿交替作用下水稻土DNRA菌的响应特征 |
| Response characteristics of DNRA bacteria in paddy soil under wet and dry alternation |
| 投稿时间：2024-09-05 |
| DOI：10.11654/jaes.2024-0745 |
| 中文关键词: 水稻土 干湿交替 DNRA菌 nrfA 群落结构 |
| 英文关键词: paddy soil alternating wet and dry DNRA bacterial nrfA community structure |
| 基金项目:湖南省教育厅重点项目（23A0185）；长沙市自然科学基金项目（kq2208129） |
|
| 摘要点击次数: 1241 |
| 全文下载次数: 1084 |
| 中文摘要: |
| 为研究不同施肥水稻土DNRA菌群落结构对干湿交替的响应，分析土壤DNRA菌对水分变化的响应特征，以长期定位试验点水稻土为研究对象，设置单施化肥（NPK）、30%有机肥+70%化肥（OM）处理，并以不施肥（CK）为对照，开展不同水分管理条件下（持续湿润、持续淹水、干湿交替）的培养试验。培养23 d后提取土壤DNA，对DNRA功能基因nrfA进行高通量测序。结果表明：培养结束后各处理水稻土DNRA菌群落组成发生了一定变化，在门水平上，干湿交替降低了Pseudomonadota、Verrucomicrobiota和 Chloroflexota 的相对丰度，提高了 Acidobacteriota 的相对丰度；持续淹水有利于 Chloroflexota 相对丰度的提高，但降低了Actinomycetota 的相对丰度；持续湿润处理下 Actinomycetota 的相对丰度较高。群落 α 多样性分析表明，干湿交替降低了土壤DNRA菌的Chao1指数、Shannon指数和Simpson指数，但持续淹水土壤的Shannon指数和Simpson指数高于持续湿润处理。相同水分管理条件下，不同施肥处理之间水稻土 DNRA 菌的 Shannon 指数无显著性差异。主成分分析结果表明，不同水分处理水稻土DNRA菌群落结构差异明显。水稻土DNRA菌群落结构和α多样性响应了水分变化，但α多样性对不同施肥方式的响应不显著，因此推测水分管理对DNRA菌的影响程度大于施肥管理。 |
| 英文摘要: |
| This paper aims at studying the response of DNRA bacteria community structure to dry and wet alternation in paddy soils with different fertilizers, and to analyze the response characteristics of soil DNRA bacteria to moisture management. Using paddy soil from longterm positioning test site, three treatments of single application of chemical fertilizer（NPK）, 30% organic fertilizer + 70% chemical fertilizer（OM）, and no fertilizer（CK）as control were selected with incubation experiments under alternating wet and dry（continuous wetting, continuous flooding, and alternating wet and dry）. Soil DNA was extracted after 23 d of incubation, and high-throughput sequencing of the DNRA functional gene nrfA was performed to investigate the response of the DNRA bacterial community structure to wet and dry alternation. The results showed that：the compositions and relative abundance of DNRA bacterial communities in paddy soils were changed to some extent. At the phylum level, wet-dry alternation decreased the relative abundance of Pseudomonadota, Verrucomicrobiota and Chloroflexota, and increased the relative abundance of Acidobacteriota. Continuous flooding favored the relative abundance of Chloroflexota but decreased the relative abundance of Actinomycetota. The relative abundance of Actinomycetota was higher under the continuous wetting treatment. Community α-diversity analysis showed that wet-dry alternation decreased Chao1 index, Shannon index and Simpson index of soil DNRA bacteria, but Shannon index and Simpson index were higher in continuously flooded soil than in wet treatment. Under the same water management conditions, there was no significant difference in Shannon index of DNRA bacteria in paddy soil between different fertilization treatments. The results of principal component analysis showed that the community structure of DNRA bacteria in paddy soil differed significantly among different moisture treatments. The community structure and α - diversity of DNRA bacteria in paddy soil responded to the moisture changes, but the response of α-diversity to different fertilizers was not significant. We hypothesize that the influence of moisture management on DNRA bacteria was greater than that of fertilizer management. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
