| 李滢,赵立琴,王嘉铭,陈伟业,孙晴.细菌群落促进菌渣-牛粪堆肥中非活性磷的溶解[J].农业环境科学学报,2025,44(6):1647-1657. |
| 细菌群落促进菌渣-牛粪堆肥中非活性磷的溶解 |
| Bacterial community promotes the dissolution of inactive phosphorus in the residue-cow manure compost |
| 投稿时间：2024-07-19 |
| DOI：10.11654/jaes.2024-0616 |
| 中文关键词: 堆肥 菌渣 细菌群落 磷组分 |
| 英文关键词: composting fungal residue bacterial communities phosphorus components |
| 基金项目:国家青年科学基金项目（31801905）；黑龙江省蔬菜现代农业产业技术协同创新体系资助项目（HNWJZTX202001）；黑龙江省自然科学基金联合引导项目（LH2021C066）；研究生创新科研项目（YJSCX2021-Y117） |
|
| 摘要点击次数: 1727 |
| 全文下载次数: 1511 |
| 中文摘要: |
| 为探究细菌群落对牛粪菌渣堆肥中非活性磷溶解性的影响，本研究以100%菌渣堆肥为对照组（CK），以菌渣∶牛粪=7∶3堆肥为处理组（CD），采用高通量测序和顺序提取法研究细菌群落对牛粪菌渣堆肥中Hedley磷组分水磷（H2O-P）、碳酸氢钠磷（NaHCO3-P）、氢氧化钠磷（NaOH-P）、盐酸磷（HCl-P）和残余磷（Residual-P）的影响，并分析环境因子与磷组分间的关系。结果表明：CD处理的堆体温度、总氮含量、总有机碳（TOC）含量、有效磷含量、EC值和C/N显著高于CK，但pH显著下降，堆肥产品品质得到改善。牛粪的添加影响了堆肥磷组分的转化，H2O-P含量在升温期、高温期和降温期显著提高，但在腐熟期显著降低；与CK相比，CD处理的碳酸氢钠无机磷（NaHCO3-Pi）含量显著提高，碳酸氢钠有机磷（NaHCO3-Po）含量无明显变化；添加牛粪显著降低菌渣堆肥腐熟期氢氧化钠无机磷（NaOH-Pi）含量，对氢氧化钠有机磷（NaOH-Po）和Residual-P影响较小，但能够显著抑制非活性磷（HCl-P）的增加，CD处理HCl-P的增加比CK低。添加牛粪使菌渣堆肥变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、绿弯菌门（Chloroflexi）以及假单胞菌属（Pseudomonas）、瘤胃球菌属（Ruminofilibacter）、发酵单胞菌属（Fermentimonas）的相对丰度均显著高于CK，促进非活性磷的转化。SEM模型表明添加牛粪强化了菌渣堆肥细菌群落溶解非活性磷的能力，CD处理HCl-P受pH、TOC、细菌群落α多样性和β多样性的共同调控，而CK组HCl-P主要受pH和细菌群落β多样性的影响。研究表明，添加牛粪可使菌渣堆肥快速升温，延长堆体的高温期，通过影响细菌群落结构和组成，促进HCl-P的溶解，进而提高堆肥品质。 |
| 英文摘要: |
| The objective of this study is to explore the effect of bacterial community on the solubility of inactive phosphorus in bovine manure residue compost. In this study, 100% bacterial residue compost was used as the control group（CK）and bacterial residue∶cow manure=7∶3 compost as the treatment group（CD）, and the effects of bacterial community on Hedley phosphorus components H2O-P, NaHCO3-P, NaOH-P, HCl-P and residual phosphorus（Residual-P）in cow fecal residue compost were studied by high-throughput sequencing and sequential extraction method, and the relationship between environmental factors and phosphorus components was analyzed. The results showed that the compost temperature, total nitrogen, total organic carbon（TOC）, available phosphorus content, EC value and C/N of the CD treatment were significantly higher than those of CK, but the pH decreased significantly, which improved the quality of compost products. The addition of cow manure affected the transformation of phosphorus components in compost, and the H2O-Pcontent increased significantly in the warming, high temperature and cooling stages, but decreased significantly in the decomposition stage. Compared with CK, the content of NaHCO3-inorganic P（NaHCO3-Pi）in CD treatment was significantly increased, and the content of NaHCO3-organic P（NaHCO3-Po）was not significantly changed. The addition of cow manure significantly reduced the content of NaOH-inorganic P（NaOH-Pi）during the decomposition stage of the compost, and had little effect on NaOH-organic P（NaOH-Po）and Residual-P, but significantly inhibited the increase of inactive phosphorus（HCl-P）, and the increase of HCl-P in CD treatment was lower than that of CK. The relative abundances of Proteobacteria, Firmicutes, Chloroflexi, Pseudomonas, Ruminofilibacter and Fermentimonas were significantly higher than those in CK by adding cow manure. Promotes the conversion of inactive phosphorus. The SEM model showed that the addition of cow manure enhanced the ability of the bacterial community to dissolve inactive phosphorus, and the HCl-P in the CD treatment was jointly regulated by pH, TOC, bacterial community α diversity and β diversity, while the main deformation of inactive phosphorus in the control group was affected by pH and β diversity of bacterial community. The addition of cow manure can make the compost of fungus residue rise rapidly, prolong the high temperature period of the compost, and promote the dissolution of HCl-P by affecting the structure and composition of bacterial community, thereby improving the quality of compost. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
