| 孙江涛,张闯闯,刘文婧,高贯威,左佳辉,蒋长龙,赵玉杰.梯度扩散薄膜（DGT）技术用于土壤中硝态氮提取检测[J].农业环境科学学报,2026,45(4):1056-1065. |
| 梯度扩散薄膜（DGT）技术用于土壤中硝态氮提取检测 |
| Extraction and detection method of nitrate nitrogen in soil using diffusive gradients in thin-films（DGT） |
| 投稿时间：2025-05-07 |
| DOI：10.11654/jaes.2025-0416 |
| 中文关键词: 硝态氮专性吸附树脂 梯度扩散薄膜技术 土壤硝态氮 |
| 英文关键词: nitrate-specific adsorption resin diffusive gradients in thin-films technique soil nitrate nitrogen |
| 基金项目:国家重点研发计划项目（2021YFD2000203）；中央高校基本科研业务费专项资金项目 |
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| 中文摘要: |
| 梯度扩散薄膜（DGT）技术可原位富集土壤中硝态氮，是硝态氮迁移转化规律研究的重要工具。本研究开发了一种以二甲基辛胺（DMOA）为功能基团的硝态氮专性吸附阴离子微粒径交换树脂（DMOA-PS-DVB）的制备方法，观察结果表明，DMOA-PS-DVB为中空的圆形颗粒结构，平均粒径为129.57 μm。采用DMOA-PS-DVB树脂制备的DMOA结合膜分布均匀，对硝态氮的吸附容量达到132.64 μg·cm-2。采用1 mol·L-1 NaCl溶液洗脱60 min，结合膜中硝态氮的洗脱效率达95%±2%。另外，基于DMOA结合膜制备的DMOA-DGT装置可在pH范围为3~9和离子强度小于15 mmol·L-1的条件下对硝态氮进行稳定提取。该DGT对环境中常见的干扰离子具有较强抗性，在硫酸盐、磷酸盐、氯离子浓度分别小于15、10、15 mmol·L-1的条件下吸附硝态氮不受干扰。将该DGT技术应用于9种不同类型土壤样品的硝态氮检测，结果表明本方法检测的土壤硝态氮含量与国家标准方法检测值之间具有显著的相关性，说明采用DGT法测定的硝态氮可以有效代表土壤硝态氮的含量特征。研究表明，DMOA-DGT为土壤硝态氮原位快速检测提供了新方法，在硝态氮运移规律研究及风险评估领域具有广阔的应用前景。 |
| 英文摘要: |
| Diffusive gradients in thin-films（DGT） technique enables in situ enrichment of nitrate nitrogen from soil, serving as a crucial tool for investigating its migration and transformation patterns. This study developed a novel DGT binding gel using dimethyloctylamine（DMOA）-functionalized anion exchange resin（DMOA-PS-DVB） as the adsorption material. Experimental results demonstrated that DMOA-PS-DVB exhibited monodisperse hollow spherical morphology with an average particle diameter of 129.57 μm. The DMOA binding gel structure maintaining uniform dispersion. Nitrate adsorption capacity of the gel reached 132.64 μg·cm-2 with an elution efficiency of 95%±2% achieved using 1 mol·L-1 NaCl solution for 60 min elution. This developed DGT device exhibited effective nitrate extraction across a broad pH range（3-9） and maintained functionality under ionic strength conditions below 15 mmol·L-1. Furthermore, DGT exhibited robust resistance to common environmental interfering ions, with nitrate nitrogen adsorption remaining unaffected under conditions where sulfate, phosphate, and chloride ion concentrations were below 15, 10 mmol·L-1, and 15 mmol·L-1, respectively. Finally, the DGT technique was employed for nitrate detection from nine distinct soil types. Results revealed a statistically significant correlation between nitrate contents determined by this method and those obtained using standard methods, demonstrating that the DGT approach could be applied to accurately characterize soil nitrate content. This investigation established that DMOA-DGT provided an innovative approach for in situ rapid detection of soil nitrate nitrogen, offering substantial potential for elucidating nitrate migration mechanisms and enhancing environmental risk assessment frameworks in agricultural ecosystems. |
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