| 刘朔,李海洋,黎杨凯,汤紫璇,牛世华,廖新俤,邢斯程.微塑料-抗生素复合污染对土壤噬菌体的影响[J].农业环境科学学报,2026,45(4):953-964. |
| 微塑料-抗生素复合污染对土壤噬菌体的影响 |
| Impact of microplastic-antibiotic co-pollution on soil bacteriophages |
| 投稿时间：2025-03-12 |
| DOI：10.11654/jaes.2025-0242 |
| 中文关键词: 微塑料污染 抗生素污染 噬菌体 碳水化合物活性酶 抗生素抗性基因 |
| 英文关键词: microplastics pollution antibiotic pollution bacteriophage carbohydrate-active enzymes antibiotic resistance genes |
| 基金项目:现代农业产业技术体系建设专项资金项目（CARS-41） |
| 作者 | 单位 | E-mail | | 刘朔 | 华南农业大学动物科学学院, 广州 510642 | | | 李海洋 | 华南农业大学群体微生物研究中心, 广州 510642 | | | 黎杨凯 | 华南农业大学群体微生物研究中心, 广州 510642 | | | 汤紫璇 | 华南农业大学群体微生物研究中心, 广州 510642 | | | 牛世华 | 温氏食品集团股份有限公司, 广东 云浮 527300 | | | 廖新俤 | 华南农业大学动物科学学院, 广州 510642
温氏食品集团股份有限公司, 广东 云浮 527300
广东省农用动物基因组学与分子育种重点实验室, 广州 510642
广东省猪禽养殖业重点实验室, 广州 510642 | | | 邢斯程 | 华南农业大学群体微生物研究中心, 广州 510642
广东省农用动物基因组学与分子育种重点实验室, 广州 510642
广东省猪禽养殖业重点实验室, 广州 510642 | scxing@scau.edu.cn |
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| 中文摘要: |
| 为探究土壤中微塑料与粪源抗生素的复合污染对土壤噬菌体的影响，本研究通过土壤孵育试验，结合宏基因组测序和病毒组分析，系统探究了微塑料与抗生素复合污染条件下土壤噬菌体群落的组成结构、功能特征及其对ARGs传播风险的影响情况。结果表明：微塑料与抗生素的复合污染虽未显著改变土壤噬菌体群落的组成结构，但导致噬菌体功能基因表达显著下调，其中编码糖苷水解酶等功能酶的关键基因表达量较无污染对照组降低17%~20%。同时，复合污染显著促进了多种ARGs的富集与水平转移，部分ARGs的富集程度达到对照组的215.33%。值得注意的是，在土壤碳循环过程中，复合污染进一步加剧了噬菌体介导的ARGs传播风险。通过线性回归分析发现，复合污染诱导的噬菌体功能失调直接影响了土壤理化性质，具体表现为土壤有机碳含量显著降低。研究表明，微塑料与粪源抗生素复合污染显著影响土壤噬菌体功能，同时增加抗生素抗性基因的传播风险。 |
| 英文摘要: |
| To investigate the impact of combined microplastic and manure-derived antibiotic pollution on soil phages, this study conducted soil incubation experiments, integrated with metagenomic sequencing and virome analysis, to systematically explore the composition, functional characteristics of soil phage communities, and their influence on ARGs dissemination under combined pollution conditions. The results demonstrated that while combined antibiotic-MP pollution did not significantly alter the compositional structure of soil phage communities, it led to a notable downregulation of phage functional gene expression. Key genes encoding functional enzymes such as glycoside hydrolases exhibited a 17%-20% reduction in expression compared to the uncontaminated control group. Simultaneously, combined pollution significantly promoted the enrichment and horizontal transfer of various ARGs, with the enrichment levels of some ARGs reaching 215.33% of those in the control group. Notably, in the soil carbon cycle, combined pollution further exacerbated phage-mediated ARGs transmission risks. Linear regression analysis revealed that phage dysfunction induced by combined pollution directly affected soil physicochemical properties, manifesting as a significant decrease in soil organic carbon content. The study indicates that combined pollution from microplastics and manure-derived antibiotics significantly impairs soil phage functionality, and increases the transmission risk of antibiotic resistance genes. |
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