| 覃桂妹,许仁智,刘智博,陈德倩,宋波.不同镉污染土壤对藿香蓟光合生理及亚细胞镉分布的影响[J].农业环境科学学报,2026,45(4):941-952. |
| 不同镉污染土壤对藿香蓟光合生理及亚细胞镉分布的影响 |
| Effects of cadmium-contaminated soil at different concentrations on photosynthetic physiology and subcellular cadmium distribution in Ageratum conyzoides L. |
| 投稿时间：2025-05-19 |
| DOI：10.11654/jaes.2025-0519 |
| 中文关键词: 藿香蓟 植物修复 镉 亚细胞分布 光合系统 |
| 英文关键词: Ageratum conyzoides L. phytoremediation cadmium subcellular distribution photosynthetic system |
| 基金项目:国家自然科学基金重点项目（52230006）；桂林市重点研发计划项目（20210212-1） |
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| 中文摘要: |
| 为探究藿香蓟（Ageratum conyzoides L.）在不同镉（Cd）含量处理下的光合生理及其亚细胞Cd分布特征，通过设置不同Cd含量处理的藿香蓟盆栽试验，进行叶绿素荧光参数分析及微区X射线荧光光谱（XRF）扫描，探明了藿香蓟Cd积累特征、叶片亚细胞组分中Cd的分布规律。结果显示：盛花期时各处理组藿香蓟的鲜质量、株高及地上部生物量均高于对照（CK组）（P<0.05），其中，土壤Cd含量为5.1 mg·kg-1（T1组）时地下部和地上部生物量达到最大值（P<0.05）。藿香蓟地上部Cd含量随土壤Cd含量增加显著升高，土壤Cd含量为60 mg·kg-1时地上部Cd含量高达421.19 mg·kg-1（P<0.05）。不同Cd含量处理，藿香蓟地上转运系数（TF）均大于1，Cd的提取量在100 μg·株-1以上，显著高于CK（0.32 μg·株-1，P<0.05），表现出超积累特性。Cd含量在藿香蓟叶片中的亚细胞分布为细胞壁>细胞器>可溶性组分，细胞壁是Cd的主要储存点位，占比高达61%~94%，降低了Cd对叶肉叶绿体的毒性。Cd在叶片中的原位分布易集中于叶尖与叶脉，且随叶片Cd含量增加，各组叶绿素含量均无明显变化，Fv/Fo（PS Ⅱ的潜在光化学效率）和Fv/Fm（PS Ⅱ的最大光化学量子产量）均无显著差异，说明高Cd处理对藿香蓟叶绿素含量与PSⅡ无显著影响。研究表明，藿香蓟对中高水平Cd污染土壤具有良好的耐性，且可通过亚细胞解毒机制有效隔离Cd，有效减轻了Cd对PS Ⅱ的毒性。 |
| 英文摘要: |
| Based on pot experiments, this study investigated the subcellular distribution characteristics of cadmium（Cd） and the response mechanisms of the photosynthetic system in Ageratum conyzoides L. under gradient Cd treatments. Pot experiments with varying Cd concentrations were conducted. We measured Cd accumulation traits, subcellular Cd distribution in leaves, chlorophyll fluorescence parameters, and performed micro-X-ray fluorescence（μ-XRF） mapping to analyze photosynthetic physiology and Cd distribution patterns. At the full-bloom stage, the fresh weight, plant height, and aboveground biomass of Ageratum conyzoides L. significantly exceeded those of the control（CK） group（P<0.05）, and the soil Cd content was 5.1 mg·kg-1（T1）, and the biomasss of the underground and aboveground parts reached the maximum value（P<0.05）. The Cd content in the aboveground part of plants increased significantly with the increase of soil Cd content and reached 421.19 mg·kg-1（P<0.05） when the soil Cd content was 60 mg·kg-1. Under different Cd content treatments, the aboveground transport coefficient（TF） of Ageratum conyzoides L. was >1, and the extraction amount of Cd was above 100 μg·plant-1, which was significantly higher than that of the CK（0.32 μg·plant-1, P<0.05）, showing superaccumulation characteristics. Subcellular Cd partitioning followed：the cell wall（61%-94%）>organelles > soluble fractions, identifying the cell wall as the primary Cd storage site, mitigating toxicity to mesophyll chloroplasts. μ-XRF revealed preferential Cd localization in leaf tips and veins, despite increasing leaf Cd accumulation, chlorophyll content remained stable. Elevated Fv/Fo（potential photochemical efficiency of PS Ⅱ） and Fv/Fm（maximum photochemical quantum yield of PS Ⅱ） indicated negligible Cd-induced damage to chlorophyll or PS Ⅱ function. Ageratum conyzoides L. shows high potential for in-situ remediation of moderately-to-highly Cd-contaminated soils. The study demonstrates that Ageratum conyzoides L. is applicable for in-situ remediation of moderately-to-highly Cd-contaminated soils and provides a theoretical foundation for enhancing subcellular detoxification mechanisms in hyperaccumulators. |
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