| 续绅,吴懿鑫,李宏卓,高岩.掺杂氧化铈纳米颗粒介导的种子引发提升油菜耐寒性机理[J].农业环境科学学报,2026,45(2):319-328. |
| 掺杂氧化铈纳米颗粒介导的种子引发提升油菜耐寒性机理 |
| Mechanisms of doped nanoceria seed priming enhanced cold tolerance in rapeseed |
| 投稿时间:2025-04-14 修订日期:2025-06-09 |
| DOI:10.11654/jaes.2025-0351 |
| 中文关键词: 油菜 纳米引发 低温胁迫 植物激素 胁迫记忆 |
| 英文关键词: rapeseed(Brassica napus L.) nano-priming cold stress plant hormones stress memory |
| 基金项目:江苏省农业科技自主创新基金项目(CX(23)1033);江苏省重点研发计划(现代农业)项目(BE2023312);江苏省农业科学院资环所青年联合创新项目(ZCX〔2022〕12) |
| 作者 | 单位 | E-mail | | 续绅 | 江苏省农业科学院农业资源与环境研究所, 南京 210014 农业农村部长江下游平原农业环境重点实验室, 南京 210014 江苏大学环境与安全工程学院, 江苏 镇江 212013 | | | 吴懿鑫 | 江苏省农业科学院农业资源与环境研究所, 南京 210014 农业农村部长江下游平原农业环境重点实验室, 南京 210014 | | | 李宏卓 | 江苏省农业科学院农业资源与环境研究所, 南京 210014 农业农村部长江下游平原农业环境重点实验室, 南京 210014 南京林业大学南方现代林业协同创新中心, 南京 210037 | | | 高岩 | 江苏省农业科学院农业资源与环境研究所, 南京 210014 农业农村部长江下游平原农业环境重点实验室, 南京 210014 江苏大学环境与安全工程学院, 江苏 镇江 212013 | ygao@jaas.ac.cn |
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| 中文摘要: |
| 为探究纳米引发技术在增强作物低温耐性中的应用潜力,本研究制备出两种掺杂氧化铈纳米颗粒:掺锰氧化铈纳米颗粒(Mn-CeO2 NPs)和氧化锌掺杂氧化铈纳米颗粒(ZnO-CeO2 NPs),以低温敏感型油菜品种宁杂158为对象,探究二者对油菜种子低温萌发的调控效应及生理机制。结果表明,在低温处理下,油菜种子发芽势和发芽指数分别降低18.5%和46.2%,Mn-CeO2 NPs引发对低温条件下油菜种子萌发特性均无显著影响,而ZnO-CeO2 NPs引发显著提升了种子的发芽势(提高26.9%)、发芽指数(提高11.1%)及活力指数(从760.2增加至788.1)。ZnO-CeO2 NPs引发主要通过两种途径增强油菜种子低温耐性:ZnO-CeO2 NPs引发通过显著提高种子中β-淀粉酶活性(提高31.2%),促进了贮藏淀粉的降解,使可溶性糖含量增加26.5%,为种子提供渗透保护与能量底物;激素代谢组学分析显示,ZnO-CeO2 NPs引发显著提升了低温胁迫下种子中赤霉素4(增加133.6%)和水杨酸(增加97.8%)的含量,两种激素通过协同作用打破生理休眠并启动系统抗性,从而增强了油菜低温耐性。 |
| 英文摘要: |
| To investigate the potential of nano-priming technology in enhancing crop cold tolerance, we prepared two doped cerium oxide nanoparticles:manganese-doped cerium oxide nanoparticles(Mn-CeO2 NPs)and zinc oxide-doped cerium oxide nanoparticles(ZnOCeO2 NPs). The regulatory effects and physiological mechanisms of the two materials on the germination of the cold-sensitive rapeseed variety Ningza 158 under cold stress were explored. Results showed that low temperature reduced germination potential and germination index by 18.5% and 46.2%, respectively. Mn-CeO2 NPs priming showed no significant effects on the germination characteristics of rapeseed seeds under low-temperature conditions. However, ZnO-CeO2 NPs priming significantly improved germination potential (increased by 26.9%), germination index(increased by 11.1%), and vigor index(from 760.2 to 788.1). ZnO-CeO2 NPs enhance the cold tolerance of rapeseed seeds primarily through two pathways:ZnO-CeO2 NPs significantly increased the activity of β-amylase in seeds by 31.2%, promoting the degradation of stored starch and increasing soluble sugar content by 26.5%, providing osmotic protection and energy substrates for the seeds; Hormone metabolomics analysis showed that ZnO-CeO2 NPs significantly improved the levels of gibberellin 4 (increased by 133.6%)and salicylic acid(increased by 97.8%)in seeds under cold stress. These two hormones, through synergistic effects, respectively break physiological dormancy and initiate systemic resistance, enhancing the cold tolerance of rapeseed. |
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