| 刘崇万,朱晓华,杨洪生,王明华,徐志华,任娣,孟勇.模拟水产养殖水体地西泮残留的微生物降解[J].农业环境科学学报,2025,44(7):1872-1882. |
| 模拟水产养殖水体地西泮残留的微生物降解 |
| Microbial degradation of diazepam in simulated aquaculture water |
| 投稿时间：2024-11-09 |
| DOI：10.11654/jaes.2024-0970 |
| 中文关键词: 水产养殖 地西泮 复合微生物 降解特性 响应面优化 降解动力学 |
| 英文关键词: aquaculture diazepam microbial consortium degradation characteristics response surface optimization degradation kinetics |
| 基金项目:江苏现代农业（大宗鱼类）产业技术体系建设项目（JATS〔2023〕334）；国家特色淡水鱼产业技术体系（CARS-46）南京综合试验站 |
| 作者 | 单位 | E-mail | | 刘崇万 | 江苏省淡水水产研究所, 南京 210017
江苏省水产质量检测中心, 南京 210017 | | | 朱晓华 | 江苏省淡水水产研究所, 南京 210017
江苏省水产质量检测中心, 南京 210017 | xhz824@sina.com | | 杨洪生 | 江苏省淡水水产研究所, 南京 210017
江苏省水产质量检测中心, 南京 210017 | | | 王明华 | 江苏省淡水水产研究所, 南京 210017 | | | 徐志华 | 江苏省淡水水产研究所, 南京 210017
江苏省水产质量检测中心, 南京 210017 | | | 任娣 | 江苏省淡水水产研究所, 南京 210017
江苏省水产质量检测中心, 南京 210017 | | | 孟勇 | 江苏省淡水水产研究所, 南京 210017 | |
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| 中文摘要: |
| 水体地西泮（DZP）残留会对人类健康及环境生态造成威胁。本研究在前期工作基础上，通过模拟水产养殖水体，进一步研究复合微生物（包括枯草芽孢杆菌、酵母菌、芽枝状枝孢菌、米曲霉及黑曲霉）对DZP残留的最佳降解条件及降解动力学，并在中试条件下进行验证，同时分析对水体、沉积物的理化特性以及对鳊、鲫的应激和存活率的影响。综合单因素试验、PlackettBurman试验及最陡爬坡试验和Box-Behnken设计，结果表明：水体溶解氧、环境温度、复合菌悬液投入量为影响降解DZP的主要因素，进一步确定3个因素的拟合值为：水体溶解氧8.5 mg·L-1，环境温度25℃，复合菌悬液投入量1.0%，该拟合值条件下预测绝对降解率最大为91.2%；中试条件下绝对降解率为90.5%，与模型预测的最大值相接近，较未优化前提高了15.59%。复合微生物对水体及沉积物的pH有显著影响，对其他环境参数及鳊、鲫应激和存活率均无显著影响。降解动力学分析发现，当DZP浓度为150 ng·L-1时，比降解率最大高达59.62 ng·L-1·h-1。通过优化降解条件，本研究复合微生物降解DZP能力显著提升，且不对水产养殖环境及水产动物造成负面影响，可有效应用于水产养殖水体DZP残留的治理。 |
| 英文摘要: |
| Diazepam（DZP）residues in water pose a threat to human health and environmental ecology. To improve DZP biodegradation efficiency, we studied the optimal application conditions and degradation kinetics of DZP residues in aquaculture water by DZP-degrading microbial consortium, including Bacillus subtilis, yeast, Cladosporium cladosporioides, Aspergillus oryzae, Aspergillus niger, which were selected in previous study. The degradation characteristics of DZP by microbial consortium were optimized by performing the single factor test, Plackett-Burman test, steepest ascent hill climb test, and Box-Behnken test, and the results were verified under pilot-scale conditions. At the same time, the effects of microbial consortium on the physical and chemical properties of water and sediment, as well as the stress and survival rate of aquatic animals（such as bream and crucian carp）were analyzed. The results of single factor test showed that the degradation effect of DZP was obvious by environmental temperature, dissolved oxygen, pH and the amount of composite bacterial suspension. Plackett-Burman experiment was used to determine the main factors affecting the degradation of DZP, including dissolved oxygen, environmental temperature and the amount of composite bacterial suspension. On this basis, the steepest ascent test and Box-Behnken design were used to determine the fitting values of the three main factors：dissolved oxygen 8.5 mg·L-1, ambient temperature 25 ℃, and the amount of composite bacterial suspension 1.0%. Under the condition of the fitting value, the maximum predicted absolute degradation rate was 91.2%; the above results were verified under pilot conditions, and the absolute degradation rate was 90.5%, which was close to the maximum value predicted by the model and increased by 15.59% compared with that before optimization. The application of microbial consortium only had a significant effect on the pH of water and sediment, there was no significant effects on other environmrnt parameters, as well as stress indicators and survival rates of Carassius auratus and Parabramis pekinensis. The kinetic analysis showed that the maximum specific degradation rate was 59.62 ng·L-1·h-1 when the concentration of DZP was 150 ng·L-1.The microbial consortium in this study have high DZP degradation ability and do not have negative effects on aquaculture environment and aquatic animals. It can be effectively applied to the treatment of DZP residues in aquaculture water, and provides an economical and environmentally friendly feasible solution for the improvement of aquatic product quality and safety and the restoration of water ecological environment. |
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