| 冉沁瑶,宋理洪,何冠谛,李春燕,刘鸿雁,芦艳珍,杨三维.蚯蚓生物标志物对Cd胁迫应激响应及影响因素的Meta分析[J].农业环境科学学报,2025,44(7):1743-1758. |
| 蚯蚓生物标志物对Cd胁迫应激响应及影响因素的Meta分析 |
| Meta-analysis of stress response on biomarkers in earthworms and their influencing factors under cadmium stress |
| 投稿时间：2024-06-24 |
| DOI：10.11654/jaes.2024-0531 |
| 中文关键词: Cd胁迫 蚯蚓 应激响应 生物标志物 Meta分析 |
| 英文关键词: cadmium stress earthworm stress response biomarker Meta-analysis |
| 基金项目:国家自然科学基金项目（42067028）；贵州特色杂粮优质高效科技创新人才团队建设项目（黔科合平台人才-BQW〔2024〕009）；贵州大学人才专项（贵大领军合字〔2024〕07号） |
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| 中文摘要: |
| 为系统总结蚯蚓生物标志物对Cd胁迫的应激响应及影响因素，本文使用Meta分析方法，提取国内外权威数据库中符合筛选标准的58篇文献的618例样本，量化Cd胁迫下蚯蚓生物标志物活性的效应值，分析Cd胁迫对蚯蚓生物标志物活性的毒害效应。分析表明：土壤Cd胁迫显著促进蚯蚓体内乙酰胆碱酯酶（AChE）、丙二醛（MDA）、金属硫蛋白（MT）和超氧化物歧化酶（SOD）的活性（P<0.01），同时抑制纤维素酶活性。蚯蚓生物标志物对Cd胁迫的影响受多重因素调控，包括Cd含量、土壤pH值、土壤有机质含量、蚯蚓种类、蚯蚓体质量、试验方法以及暴露时间。具体而言：Cd含量与过氧化氢酶（CAT）和纤维素酶效应值显著负相关（P<0.05），与MT效应值显著正相关（P<0.01）；土壤pH值与蚯蚓体内AChE、SOD和谷胱甘肽转移酶（GST）的效应值间存在显著负相关（P<0.05）；土壤有机质含量与SOD效应值呈极显著正相关（P<0.01），与CAT、过氧化物酶（POD）和MT效应值呈显著负相关（P<0.05）；在蚯蚓种类方面，赤子爱胜蚓（Eisenia fetida）因其敏感性而被认定为评估土壤Cd毒性的理想受体；蚯蚓体质量的增加促进了CAT、SOD及POD活性的提升（P<0.05）；蚯蚓体内多项生物标志物对Cd胁迫的复杂响应与试验方法和暴露时长紧密关联。研究表明，AChE、GST、MDA、MT、SOD及纤维素酶等生物标志物在土壤Cd污染环境中与氧化应激紧密相关，它们作为评估土壤Cd污染风险的关键指标具有显著的应用价值，采用这些生物标志物进行土壤Cd污染监测既高效快捷，又能有效降低成本和复杂性。 |
| 英文摘要: |
| In order to systematically summarise the stress response of earthworms biomarkers to cadmium stress and their influencing factors, this paper was based on Meta-analysis and extracted 618 samples from 58 literatures in authoritative databases at home and abroad that met the screening criteria, to quantify the effect values of earthworm biomarker activities under cadmium stress, and to analyse the toxic effects of cadmium stress on earthworm biomarker activities. Analyses indicated that soil cadmium stress markedly improved the activities of acetylcholinesterase（AChE）, malondialdehyde（MDA）, metallothionein（MT）, and superoxide dismutase（SOD）in earthworms （P<0.01）, while inhibiting cellulase activity. The impact of cadmium stress on biomarkers in earthworms were regulated by multiple factors, including cadmium concentration, soil pH, organic matter content in soil, species of earthworms, body weight of earthworms, experimental method and exposure duration. Specifically：Cadmium concentration was significantly negatively correlated with the effect size of catalase（CAT）and cellulase（P<0.05）, and significantly positively linked to the effect size of MT（P<0.01）. There was a marked negative correlation between soil pH and the effect size of AChE, SOD and glutathione S-transferase（GST）（P<0.05）; Soil organic matter content was highly significantly and positively correlated with the effect size of SOD（P<0.01）; and negatively correlated with the effect size of CAT, peroxidase（POD）and MT（P<0.05）. With regard to earthworm species, Eisenia fetida has been identified as an ideal receptor for assessing soil cadmium toxicity due to its sensitivity. Increased body weight of earthworms promotes CAT, SOD and POD activities（P<0.05）. The complex response of several biomarkers to cadmium stress in earthworms were closely related to the experimental method and the duration of exposure. To sum up, the research made it clear that biomarkers like AChE, GST, MDA, MT, SOD, and cellulase significantly contribute to oxidative stress in environments polluted with soil cadmium, making them valuable indicators in evaluating soil cadmium contamination risks. Implementing these biological indicators for tracking soil cadmium pollution proves to be swift and effective, diminishing both expenses and intricacy. This approach not only establishes a robust theoretical groundwork to enhance the precision, responsiveness, and extensive use of biomarkers in environmental surveillance but also holds significant importance in precisely assessing the condition of environmental health. |
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