| 林星妤,郭小可,扈茹静,徐小逊,杨占彪.叶面喷施L-半胱氨酸对小白菜镉积累及抗氧化系统的影响[J].农业环境科学学报,2025,44(5):1217-1224. |
| 叶面喷施L-半胱氨酸对小白菜镉积累及抗氧化系统的影响 |
| Effects of leaf spraying L-cysteine on cadmium accumulation and antioxidant system of Chinese cabbage |
| 投稿时间：2024-05-13 |
| DOI：10.11654/jaes.2024-0406 |
| 中文关键词: 叶面喷施 L-半胱氨酸 镉积累 小白菜 转运 |
| 英文关键词: foliar spraying L-cysteine cadmium accumulation Chinese cabbage transport |
| 基金项目:四川省自然科学基金项目（2023NSFSC0199）；四川省科技计划项目（2023YFS0483）；国家现代农业产业技术体系四川油菜创新团队项目（SCCXTD-2024-3） |
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| 中文摘要: |
| 为了探讨氨基酸对小白菜镉（Cd）积累能力的影响及其与抗氧化系统的关系，本实验采用盆栽方式对Cd中轻度污染条件下小白菜叶面喷施L-半胱氨酸溶液，测定小白菜Cd富集量及抗氧化系统等指标。结果表明，喷施L-半胱氨酸后小白菜地上部Cd含量呈下降趋势（P<0.05）。当喷施浓度为20、30 mmol·L-1时Cd从根部到地上部分的转运显著降低（P<0.05）。喷施时间对小白菜Cd含量影响显著（P<0.05），在三叶一心期（TCM）喷施浓度为30 mmol·L-1的L-半胱氨酸，小白菜地上部Cd含量降幅最大，达63.67%，且转运系数最小。此外，随着叶面喷施L-半胱氨酸浓度的增加，叶绿素含量增加；喷施L-半胱氨酸溶液后过氧化物酶（POD）活性提升，而超氧化物歧化酶（SOD）活性下降（P<0.05）；当喷施浓度为30 mmol·L-1时，小白菜中丙二醛（MDA）和谷胱甘肽（GSH）含量最大。研究表明，喷施L-半胱氨酸增加了小白菜的叶绿素含量、抗氧化系统活性，提高了小白菜对Cd的拦截能力，减少了Cd从地下部向地上部的转运，缓解了Cd对小白菜的胁迫。 |
| 英文摘要: |
| In order to explore the effect of amino acids on cadmium（Cd）enrichment and the relationship with antioxidant system of Chinese cabbage, this study employed different concentrations of L-cysteine solution to spray the leaf surface of Chinese cabbage in the mild Cd contaminated soil. The content of Cd and the index of antioxidant system of Chinese cabbage were measured. The results showed that the content of Cd in aboveground part of Chinese cabbage decreased after spraying L-cysteine（P<0.05）. When the spray concentration was 20 mmol·L-1 and 30 mmol·L-1, the transfer of Cd from the root to the aboveground part was significantly reduced（P<0.05）. The effect of spraying time on Cd content was significant（P<0.05）. When L-cysteine concentration of 30 mmol·L-1 was sprayed in three leaf singlephase（TCM）, the lowest value of Cd content in aboveground of Chinese cabbage were found, decreased by 63.67%. The transport coefficient was the lowest at this condition. In addition, with the increase of L-cysteine concentration, chlorophyll content of Chinese cabbage increased. After spraying L-cysteine, POD activity was increased, and SOD activity was decreased（P<0.05）. Spraying with 30 mmol·L-1 concentration of L-cysteine can increase the content of MDA and GSH in Chinese cabbage. The study showed that spraying Lcysteine increased the chlorophyll and antioxidant system activity of Chinese cabbage; improved the intercepting ability of Chinese cabbage to Cd, reduced the transfer of Cd from underground to aboveground, and alleviated the stress of Cd on Chinese cabbage. |
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