| 火兴宇,张宇,王常荣,齐麟,刘斌,黄雁飞,黄永春.硫化氢信号分子介导S-烯丙基-L-半胱氨酸调控水稻镉胁迫[J].农业环境科学学报,2025,44(5):1160-1168. |
| 硫化氢信号分子介导S-烯丙基-L-半胱氨酸调控水稻镉胁迫 |
| Hydrogen sulfide signaling molecules mediate S-allyl-L-cysteine to regulate cadmium stress in rice |
| 投稿时间：2024-09-05 |
| DOI：10.11654/jaes.2024-0744 |
| 中文关键词: 水稻 镉 S-烯丙基-L-半胱氨酸 硫化氢 信号分子 |
| 英文关键词: rice cadmium S-allyl-L-cysteine hydrogen sulfide signaling molecule |
| 基金项目:广西创新驱动发展专项资金项目（桂AA22036001-3）;国家自然科学基金项目（42077153） |
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| 中文摘要: |
| 土壤Cd污染不仅影响水稻的正常生长发育，而且导致Cd在水稻籽粒中累积，从而给人体健康造成潜在危害。硫化氢（H2S）作为一种重要的信号分子在调控植物重金属胁迫中发挥重要作用。本文利用水培试验研究了具有内源H2S供体功能的大蒜提取物S-烯丙基-L-半胱氨酸（SAC）调控水稻Cd胁迫的潜在机制。选用我国南方主栽水稻品种“中早35”作为试验材料喷施系列浓度SAC，结果表明：喷施0.2 mmol·L-1SAC后水稻幼苗地上部和根部H2S含量随时间延长持续增加，12 h后与对照（CK）处理相比分别显著增加44.57%和83.22%。采用荧光标记法对水稻幼苗叶片和根部的测定结果也表明H2S荧光强度与CK处理相比分别显著增加254.55%和700.25%。进一步对水稻幼苗地上部和根部H2S主要合成酶L-半胱氨酸脱硫酶（LCD）活性进行测定，结果表明，喷施0.2 mmol·L-1SAC后地上部和根部LCD活性与CK处理相比分别显著增加94.17%和41.05%。喷施0.2 mmol·L-1SAC后对水稻抗氧化酶系统的测定结果表明，水稻幼苗地上部超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性与CK处理相比分别显著增加32.36%、26.69%、24.87%，根部分别显著增加38.61%、11.99%、62.93%。地上部和根部丙二醛（MDA）含量与CK处理相比分别显著降低37.37%和28.54%。对水稻幼苗Cd含量的测定结果表明，当SAC喷施浓度达到0.2 mmol·L-1时地上部和根部Cd含量与CK处理相比分别显著降低18.07%和46.05%。研究表明，喷施SAC诱导了幼苗LCD活性，促进了水稻合成H2S信号分子，调控了水稻Cd胁迫和转运。 |
| 英文摘要: |
| Cadmium（Cd）pollution in paddy soil not only affects the normal growth and development of rice, but also brings potential harm to human health when Cd accumulates in grain. As an important signal molecule, hydrogen sulfide（H2S）plays an important role in the regulation of heavy metal stress in plants. In this study, a hydroponic experiment was used to investigate the potential mechanism of Sallyl-L- cysteine（SAC）, a garlic extract with H2S donor function, in regulating Cd stress in rice. The main rice variety“ZhongZao 35” planted in southern China was selected as the experimental material and sprayed with SAC at series concentration. The results showed that the H2S content in shoots and roots of rice seedlings continued to increase with time after spraying 0.2 mmol·L-1 SAC, which significantly increased by 44.57% and 83.22% after 12 h compared with the control（CK）treatment. The results of fluorescence labeling on the leaves and roots of rice seedlings also showed that the fluorescence intensity of H2S significantly increased by 254.55% and 700.25% compared with CK treatment. The results of L-cysteine desulfurase（LCD）activity showed that the LCD activities in shoot and root of 0.2 mmol·L-1 SAC were significantly increased by 94.17% and 41.05% compared with CK treatment, respectively. After spraying 0.2 mmol·L-1 SAC, the activities of superoxide dismutase（SOD）, peroxidase（POD）and catalase（CAT）in the shoot of rice seedlings were significantly increased by 32.36%, 26.69%, 24.87%, respectively, the activity in the root was significantly increased by 38.61%, 11.99% and 62.93%, respectively, compared with CK treatment. The content of malondialdehyde（MDA）in shoot and root was significantly decreased by 37.37% and 28.54%, respectively. The results showed that when the SAC spraying concentration reached 0.2 mmol·L-1, the Cd contents in shoot and root of rice seedlings were significantly reduced by 18.07% and 46.05%, respectively, compared with CK treatment. In conclusion, SAC spraying triggers the synthesis of H2S signaling molecules and regulates the alleviation of Cd stress in rice. |
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