| 张华胜,刘月敏,王晓睿,白滔,张骁文.对乙酰氨基酚对蛋白核小球藻胞外聚合物的影响[J].农业环境科学学报,2025,44(2):483-490. |
| 对乙酰氨基酚对蛋白核小球藻胞外聚合物的影响 |
| Response of extracellular polymers in Proteucotlea chlorella to acetaminophen |
| 投稿时间：2024-03-12 |
| DOI：10.11654/jaes.2024-0219 |
| 中文关键词: 对乙酰氨基酚 蛋白核小球藻 胞外聚合物 叶绿素a |
| 英文关键词: acetaminophen Proteucotlea chlorella extracellular polymers chlorophylla |
| 基金项目:国家自然科学基金青年科学基金项目（51908399）；农业农村部产地污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放课题（16nybchhj-3） |
| 作者 | 单位 | E-mail | | 张华胜 | 天津城建大学环境与市政工程学院, 天津市水质科学与技术重点实验室, 天津 300384
农业农村产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300384 | | | 刘月敏 | 天津城建大学环境与市政工程学院, 天津市水质科学与技术重点实验室, 天津 300384
农业农村产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300384 | lyuemn@126.com | | 王晓睿 | 天津城建大学环境与市政工程学院, 天津市水质科学与技术重点实验室, 天津 300384 | | | 白滔 | 天津城建大学环境与市政工程学院, 天津市水质科学与技术重点实验室, 天津 300384 | | | 张骁文 | 天津城建大学环境与市政工程学院, 天津市水质科学与技术重点实验室, 天津 300384 | |
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| 中文摘要: |
| 为探讨不同浓度对乙酰氨基酚（0~100 mg·L-1，AAP）对蛋白核小球藻叶绿素a（Chl-a）和胞外聚合物（EPS）的影响，通过室内模拟试验，在第0、2、4、6、8、10、12天分别取样分析蛋白核小球藻Chl-a和EPS对AAP的响应。结果表明：AAP可提高蛋白核小球藻中Chl-a含量，并促进EPS中多糖和蛋白质分泌，且该变化呈浓度依赖性。当AAP浓度为100 mg·L-1时，EPS中多糖和蛋白质含量分别由223.83、47.02 μg·mL-1提升至361.81、70.12 μg·mL-1。基于红外光谱分析EPS官能团变化发现，随AAP浓度的增加，代表C＝O的吸收峰强度明显增强。同时，C＝O位置的吸收峰发生偏移，且—OH基团吸收峰的变化趋势与其相似，表明EPS中—OH、C＝O等官能团能够与AAP发生结合。三维荧光光谱分析发现，随AAP浓度增加，类富里酸荧光强度逐渐减弱至消失，类腐植酸荧光强度逐渐增强，表明AAP能够影响蛋白核小球藻EPS化学组分的组成。研究表明，AAP可促进蛋白核小球藻Chl-a和EPS的合成，EPS中类富里酸及—OH、C＝O等官能团则在其与AAP结合过程中发挥着重要作用。 |
| 英文摘要: |
| To explore the different concentrations of acetaminophen（0-100 mg ·L-1, AAP）on chlorophyll a（Chl-a）and extracellular polymeric substances（EPS）of Proteucotlea chlorella, and the responses of Chl-a and EPS to AAP were analyzed by sampling on days 0, 2, 4, 6, 8, 10, and 12. The results showed that AAP could increase the content of chlorophyll a in Proteucotlea chlorella and promote the secretion of polysaccharides and proteins in EPS, which the change was concentration-dependent. Under the concentration of AAP with 100 mg·L-1, the content of polysaccharides and proteins in EPS increased from 223.83 and 47.02 μg·mL-1 to 361.81 and 70.12 μg·mL-1, respectively. Based on the analysis of EPS functional group changes by infrared spectroscopy, it was found that with the increase of AAP concentration, the intensity of the absorption peak representing C＝O increased significantly. At the same time, the absorption peaks at C＝O were shifted, and the absorption peaks of —OH groups had a similar trend, indicating that functional groups such as —OH and C＝O in EPS could bind to AAP. Three-dimensional fluorescence spectroscopy analysis showed that with the increase of AAP concentration, the fluorescence intensity of fulvic acids gradually decreased to disappear, and the fluorescence intensity of humic acids gradually increased, indicating that AAP could affect the composition of EPS chemical components of Proteucotlea chlorella. In conclusion, AAP can promote the synthesis of chlorophyll a and EPS in Proteucotlea chlorella algae, and the functional groups, such as fulvic acid, —OH and C＝O in EPS, may play an important role in the binding process with AAP. |
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