| 李静凯,朱丽琴,黄荣珍,刘萍渝,王金平,房焕英,侯斌.改变碳输入对亚热带针阔混交林土壤化学性质和微生物群落的影响[J].农业环境科学学报,2025,44(1):95-104. |
| 改变碳输入对亚热带针阔混交林土壤化学性质和微生物群落的影响 |
| Effects of changing carbon input on soil chemical properties and microbial communities in subtropical coniferous and broad-leaved mixed forests |
| 投稿时间：2024-02-18 |
| DOI：10.11654/jaes.2024-0155 |
| 中文关键词: 土壤微生物 有机碳输入 高通量测序 土壤化学性质 木荷与马尾松混交林 |
| 英文关键词: soil microorganism organic carbon input high throughput sequencing soil chemical property mixed forest of Schima superba and Pinus massoniana |
| 基金项目:国家自然科学基金项目（31660192）；江西省教育厅科学技术研究项目（GJJ190974） |
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| 中文摘要: |
| 为探究有机碳输入改变对土壤微生物群落特征的影响，促进亚热带针阔混交林土壤肥力提升和生态系统恢复，本研究以亚热带红壤退化地恢复形成的木荷与马尾松针阔混交林为研究对象，原位设置凋落物输入+根系输入+菌根输入（LRM，对照）、双倍凋落物输入+根系输入+菌根输入（2LRM）、凋落物去除+根系输入+菌根输入（NRM）、凋落物去除+根系去除+菌根输入（NNM）和凋落物去除+根系去除+菌根去除（NNN）5种处理，利用高通量测序技术研究混交林表层土壤微生物群落特征，并探讨其与土壤化学性质的关系。结果表明：2LRM和NNN处理显著降低了土壤酸性磷酸酶的活性，NRM、NNM和NNN处理显著降低了土壤真菌的丰富度指数和多样性指数，改变有机碳输入对细菌 Alpha多样性的影响不显著。混交林土壤优势细菌门为酸杆菌门（15.5%~40.2%）、变形菌门（23.6%~35.5%）和放线菌门（11.2%~17.7%），优势真菌门为担子菌门（32.5%~47.8%）、子囊菌门（26.9%~48.8%）和被孢菌门（12.8%~34.5%）。NRM 处理使子囊菌门的相对丰度提高了 62.1%，使担子菌门的相对丰度降低了 27.6%。NNM 处理使放线菌门和被孢菌门的相对丰度分别提高了57.8%和169.8%，使酸杆菌门和担子菌门的相对丰度分别降低了57.7%和22.7%。NNN处理使被孢菌门的相对丰度提高了61.0%。土壤有机质、蔗糖酶和多酚氧化酶是影响土壤细菌群落变化的关键因子，pH和蔗糖酶是影响土壤真菌群落的关键因子。研究表明，有机碳输入增加和减少均降低了真菌Alpha多样性，均未改变细菌Alpha多样性，地上凋落物和地下根系的同时去除对微生物群落组成影响最大。 |
| 英文摘要: |
| To investigate the impact of changes in organic carbon input on soil microbial community characteristics for enhancing soil fertility and restoring ecosystems in subtropical mixed coniferous and broad-leaved forests, in this study, we focus on the mixed conifer forest of Schima superba and Pinus massoniana formed by restoring subtropical red soil degraded land. Five treatments were set up in situ, namely, litter input + root input + mycorrhiza input（LRM, control treatment）, double litter input + root input + mycorrhiza input（2LRM）, litter removed + root input + mycorrhiza input（NRM）, litter removed + root removed + mycorrhiza input（NNM）, and litter removed + root removed + mycorrhiza removed（NNN）. High throughput sequencing technology was used to analyze the characteristics of microbial communities in the surface soil of the mixed forests and investigate their relationship with soil chemical properties. 2LRM and NNN treatments significantly decreased the activity of soil acid phosphatase, NRM, NNM, and NNN treatments decreased the richness index and diversity index of soil fungi, and the change of organic carbon input had no significant effect on bacterial alpha diversity. The dominant bacterial phyla in mixed forest soil were Acidobacteria（15.5% - 40.2%）, Proteobacteria（23.6% - 35.5%）, and Actinobacteria（11.2% - 17.7%）. The predominant fungal phyla were Basidiomycota（32.5%-47.8%）, Ascomycota（26.9%-48.8%）, and Mortierellomycota（12.8%- 34.5%）. NRM treatment increased the relative abundance of Ascomycota by 62.1% and decreased the relative abundance of Basidiomycota by 27.6%. NNM treatment increased the relative abundances of Actinobacteria and Mortierellomycota by 57.8% and 169.8% and decreased the relative abundance of Acidobacteria and Basidiomycota by 57.7% and 22.7%, respectively. NNN treatment increased the relative abundance of Mortierellomycota by 61.0%. Soil organic matter, sucrase, and polyphenol oxidase were the key factors affecting the changes in the soil bacterial community; pH and sucrase were the key factors affecting the soil fungal community. The increase and decrease of organic carbon input decreased the alpha diversity of fungi but did not change the alpha diversity of bacteria. Simultaneously removing aboveground litter and underground roots had the most significant effect on microbial community composition. |
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