| 唐茂淞,辛朗,张磊,谢俊博,王兴鹏,韩其晟,李明发.盐胁迫下滴施枯草芽孢杆菌对无膜滴灌棉花苗期生长和土壤环境的影响[J].农业环境科学学报,2024,43(12):2935-2946. |
| 盐胁迫下滴施枯草芽孢杆菌对无膜滴灌棉花苗期生长和土壤环境的影响 |
| Effects of drip application of Bacillus subtilis under salt stress on the growth and soil environment of cotton seedlings under filmless drip irrigation |
| 投稿时间：2023-12-17 |
| DOI：10.11654/jaes.2023-1080 |
| 中文关键词: 枯草芽孢杆菌 盐胁迫 无膜滴灌棉花 土壤微生物群落结构 偏最小二乘路径模型 |
| 英文关键词: Bacillus subtilis salt stress filmless drip irrigation cotton soil microbial community structure partial least squares path model |
| 基金项目:国家重点研发计划项目（2022YFD1900505）；兵团重大科技项目（2021AA003）；塔里木大学校长基金创新团队项目（TDZKC202002） |
| 作者 | 单位 | E-mail | | 唐茂淞 | 塔里木大学水利与建筑工程学院, 新疆 阿拉尔 843300
塔里木大学现代农业工程重点实验室, 新疆 阿拉尔 843300 | | | 辛朗 | 塔里木大学水利与建筑工程学院, 新疆 阿拉尔 843300
塔里木大学现代农业工程重点实验室, 新疆 阿拉尔 843300 | | | 张磊 | 塔里木大学水利与建筑工程学院, 新疆 阿拉尔 843300
塔里木大学现代农业工程重点实验室, 新疆 阿拉尔 843300 | | | 谢俊博 | 塔里木大学水利与建筑工程学院, 新疆 阿拉尔 843300
塔里木大学现代农业工程重点实验室, 新疆 阿拉尔 843300 | | | 王兴鹏 | 塔里木大学水利与建筑工程学院, 新疆 阿拉尔 843300
塔里木大学现代农业工程重点实验室, 新疆 阿拉尔 843300
农业农村部西北绿洲节水农业重点实验室, 新疆 石河子 832000
塔里木大学塔里木绿洲农业教育部重点实验室, 新疆 阿拉尔 843300
中国农业科学院西部农业研究中心, 新疆 昌吉 831100 | 13999068354@163.com | | 韩其晟 | 中国农业科学院农田灌溉研究所, 河南 新乡 453002 | hanqisheng@hist.edu.cn | | 李明发 | 新疆生产建设兵团第一师水文水资源管理中心, 新疆 阿拉尔 843300 | |
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| 中文摘要: |
| 为研究不同梯度盐胁迫条件下，滴施枯草芽孢杆菌（Bacillus subtilis，BS）对南疆无膜滴灌棉花苗期生长及土壤环境的影响机制，采用双因素裂区试验设计，主区为2种盐分梯度（S），包括低盐（2 mS·cm-1）和高盐（5 mS·cm-1），副区为3种BS剂量（B），包括不滴施BS、低剂量BS（15 kg·hm-2）和高剂量BS（30 kg·hm-2），共6个处理：S2B0、S2B15、S2B30、S5B0、S5B15和S5B30。分析了盐胁迫下滴施BS对棉花主要生长指标，以及土壤的主要酶活性、有机质含量、电导率、细菌和真菌群落结构的影响。结果表明：与低盐胁迫相比，高盐胁迫显著降低了棉花生长指标、土壤脲酶活性、蔗糖酶活性和有机质含量，导致土壤中有益微生物（Zeaxanthinibacter）的相对丰度减少。滴施BS缓解了盐胁迫对棉花生长的抑制作用，显著提高了土壤脲酶活性和有机质含量，并降低了土壤电导率，还增加了土壤中有益微生物（Nitrospira、Woeseia和Pseudogymnoascus）的相对丰度、减少了有害微生物（Phialophora）的相对丰度。与不滴施BS对照（S2B0、S5B0）相比，低剂量BS处理（S2B15、S5B15）和高剂量BS处理（S2B30、S5B30）的棉花茎干质量分别显著增加20.62%~63.99%（P<0.05）和30.85%~77.08%（P<0.05），叶干质量分别显著增加27.84%~74.73%（P<0.05）和40.83%~76.06%（P<0.05）。偏最小二乘路径模型分析结果表明，在盐胁迫条件下，BS通过影响土壤酶活性、有机质含量、电导率、土壤细菌和真菌群落结构，间接促进棉花生长指标的提高。滴施不同BS剂量对盐胁迫下南疆无膜滴灌棉花苗期生长指标的提高及盐渍化土壤环境的改良效果排序依次为30 kg·hm-2>15 kg·hm-2>不滴施。 |
| 英文摘要: |
| The aim of this study was to elucidate the mechanistic underpinnings of the influence of Bacillus subtilis（BS）application via drip irrigation on the early growth of cotton seedlings and the soil environment under varying salinity stress conditions in southern Xinjiang. The experiment was conducted using a dual-factor split-plot design, with the main plot consisting of two salt gradients（S）, including low salt（2 mS·cm-1）and high salt（5 mS·cm-1）, and the sub-plot consisting of three BS dosage levels（B）, i.e., no BS application, low BS dosage（15 kg·hm-2）, and high BS dosage（30 kg·hm-2）. There were six treatments：S2B0, S2B15, S2B30, S5B0, S5B15, and S5B30. The effects of drip application of BS under salt stress on the main growth indicators of cotton, as well as the impacts on the activities of key soil enzymes, organic matter content, conductivity, and the structure of bacterial and fungal communities was investigated. The results showed that compared to low salt stress, high salt stress significantly reduced cotton growth parameters, soil urease activity, invertase activity, and organic matter content, leading to a decrease in the relative abundance of beneficial microorganisms（Zeaxanthinibacter）in the soil. Drip application of BS alleviated the inhibitory effect of salt stress on cotton growth, significantly increased soil urease activity and organic matter content, and reduced soil electrical conductivity. It also increased the relative abundance of beneficial microorganisms（Nitrospira, Woeseia, and Pseudogymnoascus）in the soil and decreased the relative abundance of harmful microorganisms（Phialophora）. Compared to the control without BS application（S2B0 and S5B0）, low（S2B15 and S5B15）and high（S2B30 and S5B30）dosages of BS treatment significantly increased stem dry weight of cotton by 20.62%- 63.99%（P<0.05）and 30.85%- 77.08%（P<0.05）, and leaf dry weight by 27.84% - 74.73%（P<0.05）and 40.83% - 76.06%（P<0.05）, respectively. The analysis results of the partial least squares path model indicated that BS indirectly promotes the improvement of cotton growth indicators under salt stress conditions by affecting soil enzyme activity, organic matter content, conductivity, and soil bacterial and fungal community structures. The effects of different doses of BS on the improvement of cotton seedling growth indicators and the improvement of saline soil environment under salt stress in southern Xinjiang were ranked as follows：30 kg·hm-2>15 kg·hm-2>no BS. |
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