| 康茜琳,耿育红,张亚珍,张恒星,张庆明,韩颖,周磊.利用包埋技术促进微生物还原Cr（Ⅵ）的应用[J].农业环境科学学报,2024,43(12):2923-2934. |
| 利用包埋技术促进微生物还原Cr（Ⅵ）的应用 |
| Application of immobilization technology to promote microbial reduction of Cr（Ⅵ） |
| 投稿时间：2023-12-26 |
| DOI：10.11654/jaes.2023-1105 |
| 中文关键词: Cr（Ⅵ） 寡养单胞菌（Stenotrophomonas sp.） 红薯淀粉胶囊 包埋菌剂 |
| 英文关键词: Cr（Ⅵ） Stenotrophomonas sp. sweet potato starch capsules embedding microbial agents |
| 基金项目:国家重点研发计划项目（2019YFC1805900） |
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| 中文摘要: |
| 为解决Cr（Ⅵ）污染场地的微生物修复技术存在的环境适应性差、修复效率低等问题，本试验从包埋微生物菌剂制备出发，探究了红薯淀粉包埋法提升微生物Cr （Ⅵ）还原性能的效果，并以高羊茅为试供植物，评估了包埋微生物修复Cr （Ⅵ）污染土壤的效率及安全性。结果表明，与低（30 mg·kg-1）和高（100 mg·kg-1）含量的未经任何处理的Cr （Ⅵ）污染土壤（CK）相比，在试验第10天（早期）、第20天（中期）、第30天（末期），包埋菌处理（MIF，红薯淀粉胶囊包埋寡氧单胞菌处理）的Cr （Ⅵ）去除率随时间的延长而增加，游离菌处理（F，寡氧单胞菌处理）的Cr （Ⅵ）去除率随时间的延长而降低。与F相比，在低、高浓度的Cr （Ⅵ）胁迫末期，MIF的Cr（Ⅵ）去除率分别提升了43.80%、58.80%；MIF处理的土壤脲酶、蔗糖酶、过氧化氢酶活性，在低浓度下分别增加了1.50、1.57、1.79倍，在高浓度下分别增加了2.53、1.89、2.04倍。F、MIF处理的可交换态Cr占比呈现显著差异：在胁迫末期，与F相比，MIF处理的可交换态Cr占比分别降低了23.43%、20.37%，残渣态占比分别增加了22.31%、6.09%。与MIF处理相比，F处理显著增加了高羊茅地上及地下部分Cr含量（9.12、1.76倍和1.97、2.17倍）、丙二醛含量（1.38、1.32倍）、活性氧含量（1.48、1.30倍）和抗氧化酶活性（过氧化物酶1.55、1.45倍；过氧化氢酶1.37、1.59倍）。研究表明，与游离菌相比，包埋菌表现出更佳的Cr （Ⅵ）修复效果。 |
| 英文摘要: |
| The microbial remediation of Cr（Ⅵ）-contaminated sites is hindered by poor environmental adaptability and low remediation efficiency. In this study, the effectiveness of microbe-embedded sweet potato starch at reducing microbial Cr（Ⅵ）was assessed, and the efficiency and safety of embedded microbial remediation of Cr（Ⅵ）-contaminated soil was evaluated, using tall fescue as the experimental plant. The results indicate that, compared with untreated Cr（Ⅵ）- contaminated soil（CK）at low concentrations（30 mg·kg-1）and high concentrations（100 mg · kg-1）, the Cr（Ⅵ）removal rates of embedded bacterial treatment（MIF）increased over time, as evidenced by measurements made on the 10th（early）, 20th（middle）, and 30th（late）day of the experiment. In contrast, the Cr（Ⅵ）removal rates in the free bacterial treatment（F）decreased with time. Compared with the F treatment, the MIF treatment resulted in Cr（Ⅵ）removal rate increased by 43.80% and 58.80%, respectively. The soil urease, sucrase, and catalase activities of MIF increased by 1.50, 1.57 times, and 1.79 times, respectively, at low Cr concentrations, and 2.53, 1.89 times, and 2.04 times at high concentrations. The proportion of exchangeable Cr in MIF-treated soils showed significant differences：at the end of the Cr stress period, the proportion of exchangeable Cr decreased by 23.43% and 20.37%, in low- and high-Cr soils, respectively, compared with the F soils, and the proportion of residual Cr increased by 22.31% and 6.09%, respectively. Compared with MIF, F treatment significantly increased the Cr content（9.12, 1.76 times and 1.97, 2.17 times）, malondialdehyde content（1.38, 1.32 times）, reactive oxygen species content（1.48, 1.30 times）, and antioxidant enzyme activity（peroxidase 1.55, 1.45 times; peroxidase 1.37, 1.59 times）in the aboveground and underground parts of tall fescue. The results showed that, compared with free bacteria, embedded bacteria showed better Cr（Ⅵ）remediation efficiency, providing a new technical approach for improving the efficiency of microbial remediation of Cr（Ⅵ）- contaminated soil. |
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