| 张解和,姜佳慧,杨怡,章凯帆,吴彤彤,陶海升.单一及复合喹诺酮类抗生素胁迫下留兰香萌发生长的响应及耐受性分析[J].农业环境科学学报,2024,43(12):2876-2888. |
| 单一及复合喹诺酮类抗生素胁迫下留兰香萌发生长的响应及耐受性分析 |
| Analysis of the response and tolerance of Mentha spicata L. to single and complexed quinolone antibiotics |
| 投稿时间：2024-06-25 |
| DOI：10.11654/jaes.2024-0537 |
| 中文关键词: 喹诺酮类抗生素 留兰香 响应 耐受性 隶属函数 |
| 英文关键词: quinolone antibiotics spearmint response tolerance membership function |
| 基金项目:安徽高校协同创新项目（GXXT-2020-075）；大学生创新创业训练计划项目（202310368022，S202110368125，S202210368099，S202310368039） |
| 作者 | 单位 | E-mail | | 张解和 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002
皖南医学院医学影像学院, 安徽 芜湖 241002 | | | 姜佳慧 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002 | | | 杨怡 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002 | | | 章凯帆 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002 | | | 吴彤彤 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002 | | | 陶海升 | 安徽师范大学生态与环境学院, 皖江流域退化生态系统的恢复与重建省部共建协同创新中心, 安徽 芜湖 241002 | taohaish@ahnu.edu.cn |
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| 中文摘要: |
| 为探究喹诺酮类（QNs）抗生素胁迫下留兰香（Mentha spicata L.）种子萌发生长的响应及其耐受性情况，采用恒温保湿培养法，研究0（对照）、0.1、1、5、10、20 mg·L-1环丙沙星（Ciprofloxacin，CIP）、诺氟沙星（Norfloxacin，NOR）、氧氟沙星（Ofloxacin，OFL）单一及复合胁迫对留兰香种子萌发（日平均发芽率、平均发芽时间、发芽势、发芽指数和活力指数）和形态指标（胚根长、胚芽长和鲜质量）的影响。结果表明：相较于对照组，CIP、NOR胁迫下留兰香的日平均发芽率、发芽指数、活力指数、胚根长随浓度的增加先升后降，偏最小二乘结构方程模型表明萌发和形态指标呈极显著正相关（P<0.01），胚根长可作为毒性敏感指标。灰色关联度和相关性分析显示活力指数和胚根长与浓度呈极显著负相关，留兰香的耐受性与日平均发芽率、活力指数、胚根长、鲜质量和胚芽长密切相关，隶属函数和可塑性指数分析表明留兰香对抗生素的耐受性为OFL>复合>CIP>NOR。研究表明，单一及复合喹诺酮类抗生素胁迫下留兰香萌发及形态指标发生响应，可为抗生素的植物生态毒性及植物对抗生素的抗逆性评价提供重要参考。 |
| 英文摘要: |
| To investigate the response and tolerance of spearmint（Mentha spicata L.）seeds to quinolone（QNs）antibiotic stress, we evaluated the influence to spearmint germination indicators（mean daily germination, mean germination time, germination potential, germination index and vigor index）and morphological indicators（radicle length, germ length and fresh weight）under single and combined stresses of 0, 0.1, 1, 5, 10 mg·L-1 and 20 mg·L-1 ciprofloxacin（CIP）, norfloxacin（NOR）and ofloxacin（OFL）by constant temperature and moisturizing culture method. The results showed that the mean daily germination, germination index, vigour index and radicle length of M.spicata initially increased under CIP and NOR stress and then decreased with the increase of concentration compared to the control group. The partial least squares structural equation model indicated a significant positive correlation（P<0.01） between germination and morphological indices, and radicle length might serve as a reliable indicator of sensitivity to toxicity. Grey relational and correlation analysis indicated a significant negative correlation between the vitality index, radicle length and the concentration, the mean daily germination. The tolerance of spearmint was closely related to mean daily germination, vitality index, radicle length, fresh weight and germ length, and analysis of the membership function and plasticity index indicated that the tolerance of spearmint to antibiotics ranked as follows：OFL> complex > CIP > NOR. Responses of germination and morphological parameters of spearmint to single and combined quinolone antibiotics stress, this study can provide an important reference for the evaluation of plant ecotoxicity of antibiotics and plant resistance to antibiotics. |
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