| 孔维勇,王晓丽,王常荣,薛卫杰,郎耀臻,火兴宇,黄永春.叶面喷施蔗糖降低水稻幼苗镉含量机制[J].农业环境科学学报,2024,43(9):1951-1959. |
| 叶面喷施蔗糖降低水稻幼苗镉含量机制 |
| Mechanism for sucrose foliar application to reduce cadmium content in rice seedlings |
| 投稿时间：2023-12-27 |
| DOI：10.11654/jaes.2023-1108 |
| 中文关键词: 水稻 镉（Cd） 蔗糖 基因表达 氨基酸 |
| 英文关键词: rice cadmium（Cd） cane sugar gene expression amino acid |
| 基金项目:国家自然科学基金项目(42077153);广西科技重大专项(桂AA22036001-3) |
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| 中文摘要: |
| 为降低镉（Cd）在水稻中的累积，本研究利用水培实验探讨了叶面喷施蔗糖调控水稻幼苗Cd吸收和转运的潜在机制。结果表明：连续喷施3次浓度为1.0 g·L-1蔗糖后幼苗地上部和根部Cd含量分别显著降低26.7%和22.0%，丙酮酸含量分别显著增加11.8%、23.6%，α-酮戊二酸含量分别显著增加27.5%和35.3%，谷氨酸含量分别显著增加165.3%和40.3%，难溶态Cd分别显著增加63.7%和33.0%。幼苗根部与Cd吸收、转运相关的基因OsNRAMP1、OsNRAMP5、OsHMA2分别显著下调19.0%、78.8%、16.3%。此外，喷施蔗糖后水稻幼苗地上部SOD、CAT、POD活性分别显著提高66.9%、21.4%和23.6%。研究表明，喷施蔗糖促进了幼苗地上部和根部丙酮酸和α-酮戊二酸的生物合成，增加了谷氨酸生物含量。谷氨酸与根部和茎叶中Cd螯合增加了幼苗中难溶态Cd含量，减少了Cd从根部向地上部转运，缓解了Cd胁迫。 |
| 英文摘要: |
| To reduce the threat to human health caused by the accumulation of cadmium（Cd）in rice and its easy migration along the food chain. Here, we explored a potential mechanism for sucrose foliar application to regulate Cd uptake and transport in rice seedlings through hydroponic experiments. The results showed that after three consecutive sprays of 1.0 g·L-1 sucrose, the Cd content in the aboveground and root parts of seedlings significantly decreased by 26.7% and 22.0%, respectively. Concurrently, the contents of pyruvate, α-ketoglutarate, glutamic acid, and insoluble Cd, in the aboveground and root parts significantly increased by 11.8% and 23.6%, 27.5% and 35.3%, 165.3% and 40.3%, and 63.7% and 33.0%, respectively. Genes related to Cd uptake and translocation in the roots of the seedlings— OsNRAMP1, OsNRAMP5, and OsHMA2—were significantly down-regulated by 19.0%, 78.8%and 16.3%, respectively. Furthermore, after sucrose spraying, SOD, CAT, and POD activities in the aboveground parts of the rice seedlings increased significantly by 66.9%, 21.4%, and 23.6%, respectively. These results indicate that sucrose spraying promotes the glycolysis, pyruvate, and α - ketoglutarate in the aboveground and root parts of the seedlings and increases glutamic acid biosynthesis. Glutamate chelates with Cd in roots and shoots increasing recalcitrant Cd content in seedlings, reducing Cd transport from roots to aboveground parts, and alleviating Cd stress. |
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