| 苏会营,华少文,汪承润,李子航,荆子璇,王增权,赵馨怡,汪佳嘉,揣静.土壤草铵膦污染诱导赤子爱胜蚓蛋白质氧化损伤[J].农业环境科学学报,2024,43(7):1468-1474. |
| 土壤草铵膦污染诱导赤子爱胜蚓蛋白质氧化损伤 |
| Protein oxidative damage in Eisenia fetida exposed to glufosinate ammonium-polluted soils |
| 投稿时间：2024-01-20 |
| DOI：10.11654/jaes.2024-0078 |
| 中文关键词: 草铵膦（GLA） 赤子爱胜蚓 氧化损伤蛋白 解毒机制 健康风险 |
| 英文关键词: glufosinate-ammonium（GLA） Eisenia fetida oxidatively damaged protein detoxification mechanism health risk |
| 基金项目:安徽省高校自然科学研究项目（2022AH051587）；淮南师范学院校级科学研究项目（2023XJYB036）；安徽省大学生创新创业训练计划项目（S202210381102） |
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| 中文摘要: |
| 为评价施用草铵膦（GLA）可能给土壤生态环境带来的潜在风险，将制备的GLA人工污染土壤[0（对照）、10、20、40、80 mg·kg-1]于室内老化 3个月后置入赤子爱胜蚓，培养 30 d后研究蚯蚓体内蛋白质的氧化损伤及其解毒机制。结果表明：与对照组比较，20、40 mg·kg-1 GLA 污染土壤明显上调了蚯蚓体内 NADPH 氧化酶和 CAT 同工酶活性，同时促进了 GSH 的合成（P＜0.05）。当GLA浓度增至80 mg·kg-1时，SOD、APX、POD同工酶活性明显升高，而HSP70的合成和蚯蚓的体质量受到显著抑制。免疫印迹显示，在GLA浓度为10~40 mg·kg-1时，蚯蚓体内蛋白羰基化产物明显增加，诱导了潜在的解毒机制。泛素和20S蛋白酶体的蛋白丰度总体上随着土壤GLA浓度的增加趋于升高。在GLA浓度为40~80 mg·kg-1时，20S蛋白酶体、总蛋白酶、EPs同工酶和GSTs酶活性显著升高（P＜0.05）。研究表明，土壤GLA污染诱导了赤子爱胜蚓蛋白质的氧化损伤，泛素-20S蛋白酶体、EPs同工酶、总蛋白酶和GSTs是其重要的解毒机制。 |
| 英文摘要: |
| To evaluate the potential risk of glufosinate-ammonium（GLA）application in soil ecological environment, exogenously GLAcontaminated soils（0, 10, 20, 40, 80 mg·kg-1）were prepared, followed by indoor aging for 3 months. Eisenia fetida were cultured in the polluted soils for 30 days to investigate oxidatively damaged proteins and related detoxification mechanisms in the earthworms. In comparison to the blank, activities in isozymes of NADPH oxidases and catalases and synthesis of reduced glutathione were markedly upregulated in the earthworms in treatments with 20 mg·kg-1 and 40 mg·kg-1 GLA in the soils. When the concentration was increased up to 80 mg·kg-1, activities of isozymes of superoxide dismutase, ascorbate peroxidase, and guaiacol peroxidase in the earthworms were significantly elevated, whereas the synthesis of HSP70 was distinctly inhibited and weights of the earthworms decreased. Immunoblotting showed that carbonylated proteins were generated in the earthworms exposed to 10-40 mg· kg-1 GLA in the soils, activating potential detoxification mechanisms. In general, the abundance of ubiquitin and 20S proteasome tended to increase with the increase in GLA concentration in the soils. Moreover, the activities of 20S proteasome, total protease, and endopeptidase isozymes, as well as glutathione S-transferase（GST）, were significantly enhanced in response to 40-80 mg·kg-1 GLA in the soils（P＜0.05）. The GLA-contaminated soils, thus, caused oxidative damage of proteins in the earthworms. The ubiquitin-20S proteasome, endopeptidase isozymes, total protease, and GST are the important detoxification mechanisms in earthworms exposed to GLA-polluted soils. |
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