| 马文林,王安吉.接种比对猪粪中温条件产甲烷潜力的影响[J].农业环境科学学报,2023,42(8):1860-1869. |
| 接种比对猪粪中温条件产甲烷潜力的影响 |
| Effects of inoculation ratios on the biochemical methane potential of pig manure under mesophilic conditions |
| 投稿时间：2022-10-12 修订日期：2023-04-25 |
| DOI：10.11654/jaes.2022-1019 |
| 中文关键词: 厌氧消化 猪粪 接种比 产甲烷潜力 中温条件 |
| 英文关键词: anaerobic digestion pig manure inoculation ratio biochemical methane potential mesophilic condition |
| 基金项目:“十三五”国家重点研发计划课题（2017YFF0211703）；现代农业产业技术体系北京市生态循环与低碳发展创新团队项目（BAIC08-2022-SYZ04） |
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| 中文摘要: |
| 为探究不同接种比对猪粪中温产甲烷潜力（BMP）的影响，在（35.0±0.5）℃和3%初始挥发性固体（VS）浓度条件下分别按照猪粪与接种液比例为1∶2、1∶1和2∶1的接种比开展猪粪中温BMP实验，实验期间对各反应瓶的日甲烷产生量和发酵液上清液的pH、氨氮、总氮、葡萄糖等理化指标进行连续监测，并在反应结束后对不同接种比实验体系的微生物群落进行16SV4测序。各处理组甲烷产生量均为标准状况（1个标准大气压，0℃）体积。结果表明：累积甲烷产量和最大日甲烷产生量都与猪粪接种比呈显著正相关关系（P＜0.05），但不同接种比例下猪粪BMP（以VS计）无显著差异（P＞0.05）。在1∶2、1∶1和2∶1三种接种比例下猪粪的BMP分别为（252.05±5.12）、（250.07±16.57） mL·g-1和（249.65±13.69） mL·g-1，其均值为（250.59±1.28） mL·g-1；在微生物群落中，厚壁菌门、拟杆菌门与Cloacimonadota是猪粪厌氧消化系统中相对丰度最高的三类细菌，Halobacterota在产甲烷古菌中占绝对优势，变形菌门和Methanobacterium相对丰度的下降水平与接种比呈负相关关系。修正的Gompertz模型较Logistic模型更适合中温条件下猪粪的厌氧消化动力学分析。研究表明，在中温（[35.0±0.5）℃]和3%初始VS条件下，不同接种比的猪粪BMP值无显著差异（P＞0.05），从缩短BMP测试时间、提高效率考虑，建议猪粪BMP测试的接种比选取1∶2。 |
| 英文摘要: |
| This study aimed to explore the effects of different inoculation ratios on the biochemical methane potential(BMP)of pig manure under mesophilic conditions. BMP experiments were conducted at(35.0±0.5)℃ and 3% of the initial volatile solids(VS)concentration at the ratios of pig manure to inoculum solution of 1:2, 1:1 and 2:1, respectively. During the experiment, the methane productions of each test were monitored daily, and the physical and chemical indices of the supernatant of fermentation liquid, such as pH, ammonia nitrogen, total nitrogen, and glucose, were measured once every two to three days. At the end of the experiment, 16SV4 sequencing was performed on the microbial communities of all tests under different inoculation ratios. Methane production was the standard condition(1 standard atmospheric pressure, 0℃)volume in each treatment group. The results showed that the cumulative methane production and maximum daily methane production were positively correlated with the pig manure inoculation ratio(P＜0.05); however, there was no significant difference in the BMP(calculated by VS)of pig manure under different inoculation ratios(P＞0.05). The BMPs of pig manure were(252.05± 5.12),(250.07±16.57)mL·g-1, and(249.65±13.69)mL·g-1 at inoculation ratios of 1:2, 1:1, and 2:1, respectively. The mean BMP was calculated as(250.59±1.28)mL·g-1. Firmicutes, Bacteroides, and Cloacimonadota were the three types of bacteria with the highest relative abundance in the anaerobic digestion system of pig manure. Halobacterota dominated the methanogenic archaea. The decreasing relative abundance levels of Proteobacteria and Methanobacterium were negatively correlated with the inoculation ratio. The modified Gompertz model was more suitable than the Logistic model for anaerobic digestion kinetics analysis of pig manure under mesophilic conditions. In conclusion, there was no significant difference in BMP values of pig manure with different inoculation ratios under mesophilic conditions ([35.0±0.5)℃] and initial VS level(3%). Selecting the inoculation ratio of 1:2 for the BMP test of pig manure is recommended to shorten its measurement time and improve its working efficiency. |
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