| 郜道玉,崔华威,周源,龚萍,陈洁,万平民,邵志勇,华娟,李鹏,金尔光.翻堆频次对奶牛粪污异位发酵床中抗生素抗性基因及细菌群落的影响[J].农业环境科学学报,2023,42(8):1841-1851. |
| 翻堆频次对奶牛粪污异位发酵床中抗生素抗性基因及细菌群落的影响 |
| Effects of turning frequency on antibiotic resistance genes and bacterial communities in dairy manure ectopic fermentation bed |
| 投稿时间：2022-10-26 修订日期：2023-02-23 |
| DOI：10.11654/jaes.2022-1070 |
| 中文关键词: 奶牛粪污 异位发酵床 抗生素抗性基因 细菌群落 |
| 英文关键词: manure and sewage of dairy cattle ectopic fermentation bed antibiotic resistance genes bacterial communities |
| 基金项目:西藏自治区重点科技计划项目（XZ201801NB34）；武汉市农业科学院协同创新专项（XTCX2020002，XTCX2021002） |
| 作者 | 单位 | E-mail | | 郜道玉 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208
长江大学动物科学学院, 湖北 荆州 434025 | | | 崔华威 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208
华中农业大学动物医学院, 武汉 430070 | | | 周源 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 龚萍 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 陈洁 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 万平民 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 邵志勇 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 华娟 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | | | 李鹏 | 长江大学动物科学学院, 湖北 荆州 434025 | lipeng@yangtzeu.edu.cn | | 金尔光 | 武汉市农业科学院畜牧兽医研究所, 武汉 430208 | 523229599@qq.com |
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| 中文摘要: |
| 为了探讨翻堆频次对异位发酵床动态堆肥过程中抗生素抗性基因（Antibiotic resistance genes，ARGs）、整合子基因（intI1）及细菌群落的影响，设置1 d翻堆1次（T1）和2 d翻堆1次（T2） 2种条件，采取qPCR、16S rRNA技术研究了目标基因（tetG、tetW、sul1、sul2、blaTEM-1、ermQ和intI1）及细菌群落的动态变化，并分析ARGs与细菌群落及intI1的关系。结果表明：堆肥后T1和T2组目标基因总相对丰度较0 d时分别降低82.33%和80.46%，其中tetG、tetW、sul1、blaTEM-1、ermQ、intI1相对丰度分别降低16.70%、87.88%、54.60%、＞99.99%、97.80%、59.33%和61.33%、99.46%、50.91%、99.29%、82.23%、99.92%。网络分析发现，Trichococcus、Aquabacterium和Clostridiaceae_Clostridium等是ARGs和intI1的共同潜在宿主菌；冗余分析显示，细菌群落解释了ARGs变化的70.07%，intI1解释了25.10%。研究表明，细菌群落的演替和intI1相对丰度的变化可能是影响异位发酵床堆肥过程中ARGs相对丰度变化的关键因素，两处理组均能降低大部分ARGs的丰度，但不能有效去除tetG、sul1、sul2，其中2 d翻堆1次效果更好。 |
| 英文摘要: |
| To investigate the effect of turning frequency on antibiotic resistance genes(ARGs), integrator genes(intI1), and bacterial communities during dynamic composting in ectopic fermentation beds, two conditions were established:turning over once per day(T1 group) and turning over once every two days(T2 group). The dynamic changes of target genes(tetG, tetW, sul1, sul2, blaTEM-1, ermQ, and intI1) and bacterial communities were studied using qPCR and 16S rRNA, and the relationship between ARGs, bacterial communities, and intI1 was analyzed. The results showed that the total relative abundance of target genes in T1 and T2 groups decreased by 82.33% and 80.46%, respectively, compared with 0 d after composting. The relative abundance of tetG, tetW, sul1, blaTEM-1, ermQ, and intI1 decreased by 16.70%, 87.88%, 54.60%, ＞99.99%, 97.80%, 59.33% in T1 and 61.33%, 99.46%, 50.91%, 99.29%, 82.23%, 99.92% in T2, respectively. Network analysis revealed that Trichococcus, Aquabacterium, and Clostridiaceae_Clostridium were common potential host bacteria for ARGs and intI1. Redundancy analysis showed that the bacterial communities accounted for 70.07% of the variation in ARGs, and intI1 explained 25.10%. The result showed that the succession of bacterial communities and the change in relative abundance of intI1 may be the key factors affecting the change in relative abundance of ARGs during the composting process of the ectopic fermentation bed, and the abundance of most ARGs could be reduced under both treatments. The removal efficiency of most ARGs was better in the condition of turning over once every two days; however, tetG, sul1 and sul2 could not be effectively removed. |
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