| 于淑豪,翟中葳,沈丰菊,梁军锋,张克强,李明堂,王锐.硫氧化菌筛选及生物氧化特征研究[J].农业环境科学学报,2022,41(10):2287-2297. |
| 硫氧化菌筛选及生物氧化特征研究 |
| Screening of sulfur-oxidizing bacteria and biological oxidation characteristics |
| 投稿时间：2022-05-10 修订日期：2022-08-17 |
| DOI：10.11654/jaes.2022-0474 |
| 中文关键词: 畜禽养殖 硫化氢 除臭 硫氧化菌 盐单胞菌 |
| 英文关键词: livestock and poultry production hydrogen sulfide deodorization sulfur-oxidizing bacteria Halomonas |
| 基金项目:中国农业科学院科技创新项目;中央级科研院所基本科研业务费专项(2022-jbkyywf-ljf) |
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| 中文摘要: |
| 为减控畜禽养殖场硫化氢恶臭气体排放，以菌株S2-氧化性能及其在粪污中的硫化氢减排效果双重指标为标准筛选高效硫氧化菌，并研究其生物氧化特征及相关影响因素。结果表明：从奶牛场污水贮存池中分离得到一株高效异养硫氧化菌，该菌株S2-氧化能力较强，对奶牛场污水中硫化氢的减控率可达47.1%。经鉴定该菌株属于盐单胞菌属（Halomonas），命名为Halomonass p.AEB2。以单因素试验为基础，采用Box–Behnken响应面法优化了菌株的S2-氧化环境参数，结果显示各参数影响作用大小为温度>pH＞转速，最佳环境参数为温度32.5℃、转速210 r·min-1、初始pH 8.2。菌株AEB2在S2-负荷为100、200、500、1 000 mg·L-1时的去除率均可达到99%以上，OPED及PFOPD模型拟合结果表明，菌株AEB2在各负荷条件下的氧化速率常数分别为1.36、0.83、0.38、0.19 h-1。研究表明，菌株AEB2具有较高的S2-氧化能力，可作为硫化氢恶臭去除工程应用菌株。 |
| 英文摘要: |
| To reduce and control malodorous hydrogen sulfide gas emissions from livestock and poultry farms, we screened a highefficiency sulfur-oxidizing bacterium according to two indicators： S2- oxidation performance and hydrogen-sulfide-emission reduction in manure. In addition, we studied the biological oxidation characteristics and influencing factors of the strain. The results showed that we had isolated a highly efficient heterotrophic sulfur-oxidizing strain from the sewage storage tank of a dairy farm, which had a strong S 2- oxidation capacity and reduced the hydrogen sulfide in the pasture sewage by 47.1%. We identified this strain as Halomonas, which we named Halomonas sp. AEB2. Based on the results of single-factor tests, we optimized the environmental S2- oxidation parameters of strain AEB2 using the Box–Behnken response surface methodology. The results showed that the factors affecting the sulfide removal rate were temperature > pH > rotational speed, and the optimal environmental parameters were a temperature of 32.5 ℃, speed of 210 r·min-1, and initial pH of 8.2. The sulfide removal rate of strain AEB2 was more than 99% when the S2- load was 100, 200, 500 mg·L-1, and 1 000 mg· L-1; the fitting results of the OPED and PFOPD models showed that the oxidation rate constants of strain AEB2 under the various loading conditions were 1.36, 0.83, 0.38 h-1, and 0.19 h-1, respectively. AEB2 has a high S2- oxidizing ability and can be used as an engineered bacterial strain for hydrogen-sulfide-odor removal. |
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