| 罗秋萍,李虎,苏建强.酚醛泡沫对蚯蚓及土壤微生物活性的影响[J].农业环境科学学报,2022,41(5):976-982. |
| 酚醛泡沫对蚯蚓及土壤微生物活性的影响 |
| Effects of phenolic foam on earthworms and soil microbial activities |
| 投稿时间：2021-09-27 |
| DOI：10.11654/jaes.2021-1120 |
| 中文关键词: 酚醛泡沫 赤子爱胜蚓 抗氧化物酶 土壤酶活性 土壤呼吸速率 |
| 英文关键词: phenolic foam Eisenia fetida antioxidant enzyme soil enzyme activity soil respiration |
| 基金项目:国家自然科学基金项目（U1805244） |
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| 摘要点击次数: 1650 |
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| 中文摘要: |
| 为检测市面上某种农用酚醛泡沫的使用安全性，采用人工土壤法研究酚醛泡沫对赤子爱胜蚓的急性毒性；同时测定了酚醛泡沫暴露64 d后的慢性毒性效应，对蚯蚓超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性的影响，并分析了土壤中性磷酸酶（S-NP）、脲酶（S-UE）活性和呼吸速率的变化，探讨了酚醛泡沫对土壤活性及功能的影响。结果表明:酚醛泡沫对赤子爱胜蚓的急性半致死浓度大于5 000 mg·kg-1；酚醛泡沫（1~5 000 mg·kg-1）处理64 d后，蚯蚓GSH-Px活性变化范围为27.47~56.96U·mg-1，SOD活性变化范围为32.43~58.10 U·mg-1，两种酶活性均显著增加（P<0.05）。不添加酚醛泡沫的对照处理土壤呼吸速率为0.067 μmol·g-1·h-1，添加酚醛泡沫的处理组为0.041~0.055 μmol·g-1·h-1，表明酚醛泡沫添加显著（P<0.05）抑制了土壤呼吸；但酚醛泡沫处理对土壤S-NP和S-UE活性没有显著影响。研究表明，酚醛泡沫材料（5 000 mg·kg-1）对蚯蚓没有致死作用，但会诱导蚯蚓酶活性显著增强，抑制土壤呼吸速率。 |
| 英文摘要: |
| This study explored the effects of phenolic foam on Eisenia fetida and microbes in soils. In this study, we added different concentrations of phenolic foam to an artificial soil and investigated the survival and superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）enzyme activities of E. fetida. Meanwhile, soil respiration and neutral phosphatase（S-NP）and urease（S-UE） enzyme activities were recorded following phenolic foam addition to determine the chronic effects of phenolic foam on the activities of soil microbes. The results revealed that the half-lethal concentration of phenolic foam was higher than 5 000 mg·kg-1. Moreover, in soils treated with 1~5 000 mg·kg-1 phenolic foam, the levels of GSH-Px activity of E. fetida ranged from 27.47 U·mg-1 to 56.96 U·mg-1, whereas those of SOD activity of E. fetida ranged from 32.43 U · mg-1 to 58.10 U · mg-1. In addition, GSH-Px and SOD activities in soils treated with phenolic foam were significantly higher（P<0.05）than those in the control soil, indicating the occurrence of an anti-oxidation response to granular phenolic foam in E. fetida. Soil respiration rates ranged from 0.041 μmol·g-1·h-1 to 0.055 μmol·g-1·h-1, and granular phenolic foam significantly inhibited soil respiration（P<0.05）. However, phenolic foam treatment triggered no significant differences in S-NP and S-UE activities. In summary, phenolic foam had no lethal effect on E. fetida, but caused oxidative stress in this species and reduced soil respiration. |
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