| 姜毅,汤鼎,周宇诚,丁聪聪,赵兴青,柴育红.Bacillus sp.T124对六价铬的生物还原[J].农业环境科学学报,2022,41(5):967-975. |
| Bacillus sp.T124对六价铬的生物还原 |
| Bioreduction of Cr（Ⅵ）by Bacillus sp. T124 |
| 投稿时间：2021-09-09 |
| DOI：10.11654/jaes.2021-1046 |
| 中文关键词: 铬 芽孢杆菌 生物还原 共存离子 环境修复 |
| 英文关键词: chromium Bacillus bioreduction coexisting ions environmental remediation |
| 基金项目:国家自然科学基金项目（41302025，41541016）；国家自然科学基金重大研究计划项目（92062213） |
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| 中文摘要: |
| 为进一步了解菌株还原Cr （Ⅵ）的机理，从矿区周边重金属污染土壤中筛选出一株对Cr耐受的菌株Bacillus sp.T124，对该菌株去除Cr （Ⅵ）的效率和Cr （Ⅵ）的还原产物进行了研究。结果表明：Bacillus sp.T124可以有效去除Cr （Ⅵ），在Cr （Ⅵ）初始浓度为100 mg·L-1的LB培养基中48 h还原率达到43.2%。此外，在作为菌体电子供体的6种碳源（葡萄糖、果糖、蔗糖、乳糖、乙酸钠和甘露醇）中，果糖对Cr （Ⅵ）的还原效果最好，在不同共存离子存在条件下，HCO3-的添加对Cr （Ⅵ）的生物还原抑制作用最明显。扫描电镜（SEM）的结果显示，经Cr （Ⅵ）处理后的细胞形态未发生明显变化；X射线衍射（XRD）和拉曼图谱的分析表明菌株对Cr （Ⅵ）的还原产物为Cr2O3；傅里叶红外光谱（FT-IR）的结果表明还原过程有烷基和羧基以及多糖的参与，并且通过X射线电子能谱（XPS）的分析确定了去除Cr （Ⅵ）的途径为生物还原而非生物吸附。研究表明，生物还原是Bacillus sp.T124去除Cr （Ⅵ）的主要途径，碳源和共存离子是Bacillus sp.T124对Cr （Ⅵ）还原过程中的关键因素。 |
| 英文摘要: |
| To better understand the mechanism of reducing Cr（Ⅵ）, A Cr tolerant strain Bacillus sp. T124 from a heavy metal polluted soil around a mining area was screened; the efficiency of removing Cr（Ⅵ）, and the reducing products of the strain were studied. Results showed that Bacillus sp. T124 could effectively remove Cr（Ⅵ）, and the reduction rate was 43.2% in LB medium with an initial concentration of 100 mg·L-1 of Cr（Ⅵ）for 48 h. In addition, among the bacterial electron donor for Cr（Ⅵ）reduction, fructose was the most effective of the six carbon sources（glucose, fructose, sucrose, lactose, sodium acetate, and mannitol）Cr（Ⅵ）, and the addition of HCO3- had the most obvious inhibitory effect on the biological reduction of Cr（Ⅵ）. Scanning electron microscope（SEM）results showed no significant changes in the morphology of the cells treated with Cr（Ⅵ）. Diffraction of X-rays（XRD）and Raman spectra revealed that the reduction product of Cr（Ⅵ） was Cr2O3. Fourier transform infrared spectrometer（FT-IR）results showed that alkyl and carboxyl groups and polysaccharides were involved in the reduction process, and X-ray photoelectron spectroscopy（XPS）analysis suggested that the way to remove Cr（Ⅵ）was bioreduction rather than biosorption. These results showed that bioreduction was the main process that Bacillus sp. T124 use in removing Cr （Ⅵ）, and carbon source and coexisting ions were the key factors involved in the process of Cr（Ⅵ）removal by Bacillus sp. T124. |
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