李茂林,陈喆,周红燕,吴佳玲,李侃麒,游少鸿.天然有机酸强化伴矿景天提取镉的潜力与机制[J].农业环境科学学报,2022,41(4):774-784. |
天然有机酸强化伴矿景天提取镉的潜力与机制 |
Cadmium remediation efficiency and mechanism of enhanced phytoextraction by Sedum plumbizincicola with natural organic acids |
投稿时间:2021-08-28 |
DOI:10.11654/jaes.2021-0978 |
中文关键词: 伴矿景天 Cd 土壤 柠檬酸 苹果酸 组培苗 扦插苗 |
英文关键词: Sedum plumbizincicola Cd soil citric acid malic acid tissue culture plantlet seedling cutting |
基金项目:国家自然科学基金青年科学基金项目(41807136);广西科技基地和人才专项(桂科AD19110012);广西环境污染控制理论与技术重点实验室开放基金项目(桂科能1701K006);漓江流域重金属污染农田生态修复与景观利用关键技术开发与应用项目(20190219-3) |
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中文摘要: |
为探究天然有机酸对伴矿景天吸收和富集Cd的影响,采用土培盆栽试验方法,以伴矿景天组培苗和扦插苗作为2种供试景天苗,并设计不施强化剂处理和基施5 mmol·kg-1柠檬酸、基施5 mmol·kg-1苹果酸2种强化剂施用处理,监测了土壤pH、有效态Cd含量和伴矿景天生物量、Cd含量及根系微区Cd2+吸收速率。结果表明:与不施强化剂的对照组相比,基施5 mmol·kg-1柠檬酸或苹果酸均可以降低土壤pH,提高土壤有效态Cd含量,有效促进伴矿景天的生长以及提高根部微区Cd2+吸收速率,分别使伴矿景天组培苗地上部Cd富集量提高了90.4%和61.6%,扦插苗地上部Cd富集量提高了73.8%和34.5%。强化方式相同时,伴矿景天扦插苗的生物量和地上部Cd含量显著高于组培苗。研究表明,基施5 mmol·kg-1柠檬酸是强化伴矿景天修复Cd污染农田土壤的有效方法,扦插苗在Cd污染土壤的生长适应性与修复效率方面较组培苗表现得更好。 |
英文摘要: |
A pot experiment was conducted to investigate the enhanced phytoextraction of cadmium(Cd)in tissue culture plantlets and seedling cuttings of Sedum plumbizincicola using 5 mmol·kg-1 citric acid, 5 mmol·kg-1 malic acid, or 0 mmol·kg-1 organic acid(control). We measured soil pH, available Cd, and Cd accumulation in specific plant organs. Net Cd fluxes in the meristematic zone, growth zone, and maturation zone of roots were monitored using non-invasive micro-test technology. Compared to the control, soil application of 5 mmol·kg-1 citric acid or malic acid significantly increased the available Cd in the soil as well as net Cd influxes in three root micro-zones by acidizing the soil. Both citric and malic acid enhanced Sedum growth and Cd content in the plant organs. The Cd content in the shoots of Sedum plantlets increased by 90.4% and 61.6%, respectively. The Cd content in the shoots of seedling cuttings increased by 73.8% and 34.5%, respectively. The application of 5 mmol · kg-1 citric acid can effectively enhance phytoremediation of Cd-contaminated soil with S.plumbizincicola. Compared with tissue culture plantlets, seedling cuttings have better growth adaptability and remediation efficiency in Cdcontaminated soil. |
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